How can i separate functional and nonfunctional enzyme or proteins for produce a pure and functional enzyme or protein?

03 April 2019 332 3 View

Time of the GC or TA Melting Temperature?

how we can calculate Melting Temperature time for GC and TA and a primer ? I want time of this process for each GC and AT and our primers .

03 April 2019 3,571 2 View

Which books or videos or studeis for syntetich biology?

I am very interested in the field of synthetic biology and I intend to begin studies on this subject. But I cant find the right way, How do I do my research that does not eliminate my...

03 April 2019 7,972 1 View

What is the best way to maintain a plasmid structure without any nucleotide changes?

After each replication of the plasmid in bacteria, it undergoes nucleotide changes. What is the best way to maintain a plasmid structure without any nucleotide changes ?

03 April 2019 1,619 4 View

What is the best way storing master mixes for months ?

The problem with freezing our mastermixs with the enzyme is that freezing certain active enzymes such as the polymerase family of enzymes tends to result in diminished or ablated enzyme...

02 March 2019 5,207 4 View

What is the best protocols for blastomere Dissociation ?

we tried dissociate blastomere of mice by trypsin but after several protocols I lost my blastomeres and I didnt find them . how can I dissociation blastomeres and generate sin gle cells without...

11 December 2018 5,534 0 View

After replication of RNA virus in host how virus detect RNA genome from host's RNA?

after replication of RNA viruses in the cytoplasm of the host, both of genome RNA and host RNA are existing in the cytoplasm but for packing of the virus , it should detect its RNA genome. How is...

02 March 2018 5,715 4 View

What is the difference between ESCs and ESCs cell line?

What is the difference between ESCs and ESCs cell line? Is there any difference in expression of genes and differentiation and survival? What is the point to be titled?

01 February 2018 7,052 0 View

What is the best dyes for finding several viruses in a sample for use in Real Time PCR?

I want to detect several viruses using Real-Time PCR simultaneously. What is the best probes and dyes attached to them?

01 February 2018 2,947 2 View

Are there Any RdRp( Rna dependent Rna polymerase) with repair ability and without mutagenesis ?

Are there Any RdRp( Rna dependent Rna polymerase) with repair ability and without mutagenesis ? RdRp related to negative sense RNA viruses

31 December 2017 5,884 4 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How to dissolve lyophilized silk fibroin?

Dear members of the community, I am trying to concentrate silk fibroin to 25 % (w/v). Nevertheless, i am struggling with the concentration step. I successfully achieve concentrations of 5-6 % of...

16 February 2021 1,808 1 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

How to determine the drug loading and the drug release capacity of a polymer micelle using UV spectroscopy?

Currently, I am working on PLA-PEG copolymer micelle for the drug delivery system. I have seen several papers where some researcher talks to centrifuge the drug-loaded micelle to separate the...

13 February 2021 5,822 7 View

PV system design. 3d modelling and Analysis?

I have been looking for a good cost-effective tool for 3D modelling a glass-glass solar module system. I was initially considering Skelion as a tool but it does not look very promising, a bit...

13 February 2021 8,126 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

Extraction of phytochemicals from juice?

I am trying to extract phytochemicals from cane juice. I have tried lyophilization and SALLE techniques but the final product is too sticky and sugars are hindering any further extraction. I have...

03 February 2021 8,784 3 View