How can i separate functional and nonfunctional enzyme or proteins for produce a pure and functional enzyme or protein?

03 April 2019 332 3 View

Time of the GC or TA Melting Temperature?

how we can calculate Melting Temperature time for GC and TA and a primer ? I want time of this process for each GC and AT and our primers .

03 April 2019 3,571 2 View

Which books or videos or studeis for syntetich biology?

I am very interested in the field of synthetic biology and I intend to begin studies on this subject. But I cant find the right way, How do I do my research that does not eliminate my...

03 April 2019 7,972 1 View

What is the best way to maintain a plasmid structure without any nucleotide changes?

After each replication of the plasmid in bacteria, it undergoes nucleotide changes. What is the best way to maintain a plasmid structure without any nucleotide changes ?

03 April 2019 1,619 4 View

What is the best lyophilization method in lab with low cost ?

I want lyophilized master mix with enzyme. what is the best lyophilization method in lab with low cost ?

02 March 2019 9,467 2 View

What is the best way storing master mixes for months ?

The problem with freezing our mastermixs with the enzyme is that freezing certain active enzymes such as the polymerase family of enzymes tends to result in diminished or ablated enzyme...

02 March 2019 5,207 4 View

What is the best protocols for blastomere Dissociation ?

we tried dissociate blastomere of mice by trypsin but after several protocols I lost my blastomeres and I didnt find them . how can I dissociation blastomeres and generate sin gle cells without...

11 December 2018 5,534 0 View

After replication of RNA virus in host how virus detect RNA genome from host's RNA?

after replication of RNA viruses in the cytoplasm of the host, both of genome RNA and host RNA are existing in the cytoplasm but for packing of the virus , it should detect its RNA genome. How is...

02 March 2018 5,715 4 View

What is the difference between ESCs and ESCs cell line?

What is the difference between ESCs and ESCs cell line? Is there any difference in expression of genes and differentiation and survival? What is the point to be titled?

01 February 2018 7,052 0 View

What is the best dyes for finding several viruses in a sample for use in Real Time PCR?

I want to detect several viruses using Real-Time PCR simultaneously. What is the best probes and dyes attached to them?

01 February 2018 2,947 2 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View