Coconut water is the liquid present naturally inside the young fresh coconuts where as coconut milk is obtained by treating the fresh grated coconut meat with hot water. Coconut water is rich in potassium, cytokinins , various minerals and antioxidants.Whereas coconut milk is rich in fats and flavanoids.

Tasneem Gandhi is right, generally in plant tissue culture coconut water is used as a natural source of Cytokinins and other nutrients.In my lab we use coconut water in medium for invitro propagation of orchids.

Coconut water is the liquid present naturally inside the young fresh coconuts where as coconut milk is obtained by treating the fresh grated coconut meat with hot water. Coconut water is rich in potassium, cytokinins , various minerals and antioxidants.Whereas coconut milk is rich in fats and flavanoids.

Tasneem Gandhi is right, generally in plant tissue culture coconut water is used as a natural source of Cytokinins and other nutrients.In my lab we use coconut water in medium for invitro propagation of orchids.

Dear,

You can use coconut water which can supplement cytokinins as well as other nutrients You can always perform a comparative analysis between two of these. But, always do a perfect filter sterilization.

Regards

Nayem Zobayer

coconut water

I dont advise using cocnut water on orchid cultivation...

Coconut water is a very good nutrient rich medium for plant tissue culture. if it is collected under sterile conditions there is no need for further sterilization for this solution

Thank you

There is a publication from CSIR-IHBT by Dr. PS Ahuja. Refer that publication.

Coconut water is useful for in vitro culture in a wide range of plants, including bryophytes.

Dear Nikhil, use of coconut water is passe. It is variable in its contents in terms of souce of coconut growing, age, physiological status etc. Why dont you start experimenting with regular cytokinins and auxins? In that way, you will not be wasting your time.

Hi, here is a useful recent review on this topic. Best wishes.

https://www.researchgate.net/publication/40768354_The_chemical_composition_and_biological_properties_of_coconut_%28Cocos_nucifera_L.%29_water?ev=prf_pub

Article The Chemical Composition and Biological Properties of Coconu...

Here are the coconut water - fruits or canned coconut water.

Here are the dried coconut extracts (locally called "suntan" In Malaysia and Singapore) that were derived from the solid (white) endosperm of older coconuts, commonly called copra. When the "suntan" is reconstituted in water, it gives you the (white" coconut milk.

Based on our last 20 years of research and plant industrial experiences (tissue cultures of many different species), we normally used younger and green coconuts (that is nice to drink) as a source of plant growth hormones and growth stimulant. As many of you may know that coconuts are naturally available biological materials and thus cytokinins may be variable in different coconuts collected from different places, we normally collect all the coconut water (CW) from about 300 to 500 nuts, and then freeze them into one batch of CW. We then take a sample of the CW and run cytokinin-auxin-ABA and gibberallins - Multi-hormone ELISA or LC-MS to check what are we getting from this batch of CW. This allows us to do pretty decent quality monitoring, at acceptable cost, of the batch to batch variation in the types and amounts of the many growth promoting phytohormones present natural in the semi-translucent CW.

It would be nice if everyone would conform to Doctor Muhandas's definition, At the retail level with products coming from different countries they are interchangeable. Check the ingredients.

If you want to use coconut water in tissue culture please use Doctor Muhandas's definition. Coconut milk is about 18% oil and will not be particularly useful.

Many years ago I developed a tissue culture medium for Australian terrestrial orchids based on a medium used for asymbiotic germination (i.e. without mycorrhizal fungus) called Pa5. This contained coconut water but I also added micronutrients and 10 uM N6 benzyladenine. This was not particularly efficient process but did produce protocorm-like bodies which could be subcultured onto medium without coconut water and cytokinin producing roots on plantlets in vitro. A good place to start developing a medium for in vitro orchid propagation is with a complete medium that promotes the germination of the seed of the species of interest under asymbiotic conditions and modify by addition of either cytokinins or coconut water or both. The book Orchid Biology Reviews and Perspectives I.' Ed. J. Arditti.  (1977) contains a very useful chapter on clonal propagation.

The published method for Australian terrestrial orchids is: Collins, M.T. and Dixon, K.W. (1992) Micropropagation of an Australian terrestrial orchid Diuris longifolia R. Br., Australian Journal of Experimental Agriculture 32 131-5

Can anyone give me an approximate concentration of cytokinins in Coconut water ?