Nikhil Job

20 Questions 12 Answers 0 Followers

Questions related from Nikhil Job

Protein concentration in EMSA reaction?

Can anyone tell me a rough protein final concentration for a good start? I am using 20fmol biotinylated DNA in 20ul reaction volume. What can be good amount of DNA binding protein to start with?

12 December 2016 5,586 3 View

Sequences of pGWB3 vector with GUS reporter?

Can any one provide me the complete sequence of pGWB3 plant expression vector?

05 May 2015 10,112 4 View

Why does reaction volume decreases after restriction digestion of plasmid?

I have noticed that my reaction volume has been decreased by 2-3 ul after the restriction digestion of plasmids with different restriction enzymes at 37 °C. I am sure that there is no or very...

08 August 2014 8,337 4 View

How to design Real time PCR primers?

I want to design real time PCR primers for my gene of interest which is 1Kb in size and with one intron. What are the important parameters that I should keep in mind while designing primers? Is...

07 July 2014 3,439 8 View

What are the best methods to evaluate and compare the gene expression in plants?

I want to check the gene expressions of two family of plants. What are the methods that I can use? I also want to know the limitations. A discussion on this topic is expected.

11 November 2013 2,492 3 View

Which ribonucleic acid sequence gives better results in RNA interference mediated gene silencing in plants?

I would like to know which sequence (shRNA, siRNA etc) is appropriate for gene silencing in plant species? What are the factors that should be considered for selection of RNA sequence?

11 November 2013 8,709 2 View

What are the different RNA interference methods?

I would like to know more on their advantages, disadvantage and their use in gene expression analysis.

11 November 2013 7,348 0 View

What kind of expression and purification system can be used?

My protein of interest (human) contains glycosylation and having 34 kDa molecular weight. For crystallization purpose.

11 November 2013 2,956 1 View

Does anybody know the plant hormones which are secreted at the time of fruit formation in plants?

I am specifically interested in plant hormones which are either only secreted at the time of fruit formation or which are down regulated at that time. No need of specificity, can be any plant.

11 November 2013 10,000 12 View

How can I activate my oncogene of interest in plants?

I want to silence an oncogene (which is transfected) for a period of time and activate it. How can I do that? Could you suggest some papers for the above case,if any? This is a general case.

11 November 2013 3,759 1 View

What are plantibodies?

How are they different from normal antibodies? Seeking information about their generation and isolation.

10 October 2013 1,298 1 View

Are synthesized nanoparticles permeable through animal cell membranes under normal physiological conditions?

What is the the size range and chemical nature of nanoparticles which can pass through cell membranes? If it is not permeable how can we induce its permeability?

09 September 2013 8,006 6 View

Why journals are not accepting general review articles which is stating both positive and negative facts of genetically modified organisms?

I have found some journals which are publishing articles with either positive or negative impact on GMO.

08 August 2013 2,625 0 View

Is it necessary to put DOI (digital object identifier) in references while writing an article?

I have found some references without DOI. Can anyone help me ?

07 July 2013 4,896 1 View

Is there any database or a website with information about proteins with altered role in cancer cells?

I would like to find some proteins and it abnormal interactions in cancer cells.

07 July 2013 6,408 1 View

Can someone advise on the altered role of Tyrosine Phosphatases in cancer cells?

Normal interaction and altered interaction with other proteins ,and name of the proteins which is interacting with in cancer cells

07 July 2013 7,070 0 View

Is there any software (procedure) which can identify the non functioning gene?

I have the whole genomic sequence of a plant. Is there any way to find out the gene which is responsible for the survival or stress response by analyzing the closely related species of same...

07 July 2013 3,228 1 View

What is the difference between coconut milk and water? Does anybody is having experience with using them as a medium in plant tissue culture?

I would like to culture an orchid explant in vivo. Which will be good,coconut water or milk?

07 July 2013 1,517 17 View

Can anyone advise on why contamination occurs in a plant tissue culture tubes after 40 days?

How to eliminate this problem ? I am using MS medium with PGR ,and working with orchid's

07 July 2013 5,102 5 View

Which wavelength is suitable for plant regeneration from explant of orchidaceae?

Which wavelength will produce responses in plant tissue culture?

07 July 2013 7,264 1 View