I would like to know more on their advantages, disadvantage and their use in gene expression analysis.
Can anyone tell me a rough protein final concentration for a good start? I am using 20fmol biotinylated DNA in 20ul reaction volume. What can be good amount of DNA binding protein to start with?
11 December 2016 5,624 3 View
Can any one provide me the complete sequence of pGWB3 plant expression vector?
04 May 2015 10,144 4 View
I have noticed that my reaction volume has been decreased by 2-3 ul after the restriction digestion of plasmids with different restriction enzymes at 37 °C. I am sure that there is no or very...
07 August 2014 8,367 4 View
I want to design real time PCR primers for my gene of interest which is 1Kb in size and with one intron. What are the important parameters that I should keep in mind while designing primers? Is...
06 July 2014 3,516 8 View
My protein of interest (human) contains glycosylation and having 34 kDa molecular weight. For crystallization purpose.
10 November 2013 2,976 1 View
I want to silence an oncogene (which is transfected) for a period of time and activate it. How can I do that? Could you suggest some papers for the above case,if any? This is a general case.
10 November 2013 3,791 1 View
I would like to know which sequence (shRNA, siRNA etc) is appropriate for gene silencing in plant species? What are the factors that should be considered for selection of RNA sequence?
10 November 2013 8,733 2 View
I am specifically interested in plant hormones which are either only secreted at the time of fruit formation or which are down regulated at that time. No need of specificity, can be any plant.
10 November 2013 10,032 12 View
I want to check the gene expressions of two family of plants. What are the methods that I can use? I also want to know the limitations. A discussion on this topic is expected.
10 November 2013 2,508 3 View
How are they different from normal antibodies? Seeking information about their generation and isolation.
09 October 2013 1,323 1 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View