Sequences of pGWB3 vector with GUS reporter?

More Nikhil Job's questions See All

Protein concentration in EMSA reaction?

Can anyone tell me a rough protein final concentration for a good start? I am using 20fmol biotinylated DNA in 20ul reaction volume. What can be good amount of DNA binding protein to start with?

11 December 2016 5,624 3 View

Why does reaction volume decreases after restriction digestion of plasmid?

I have noticed that my reaction volume has been decreased by 2-3 ul after the restriction digestion of plasmids with different restriction enzymes at 37 °C. I am sure that there is no or very...

07 August 2014 8,367 4 View

How to design Real time PCR primers?

I want to design real time PCR primers for my gene of interest which is 1Kb in size and with one intron. What are the important parameters that I should keep in mind while designing primers? Is...

06 July 2014 3,516 8 View

What kind of expression and purification system can be used?

My protein of interest (human) contains glycosylation and having 34 kDa molecular weight. For crystallization purpose.

10 November 2013 2,976 1 View

How can I activate my oncogene of interest in plants?

I want to silence an oncogene (which is transfected) for a period of time and activate it. How can I do that? Could you suggest some papers for the above case,if any? This is a general case.

10 November 2013 3,791 1 View

Which ribonucleic acid sequence gives better results in RNA interference mediated gene silencing in plants?

I would like to know which sequence (shRNA, siRNA etc) is appropriate for gene silencing in plant species? What are the factors that should be considered for selection of RNA sequence?

10 November 2013 8,733 2 View

Does anybody know the plant hormones which are secreted at the time of fruit formation in plants?

I am specifically interested in plant hormones which are either only secreted at the time of fruit formation or which are down regulated at that time. No need of specificity, can be any plant.

10 November 2013 10,032 12 View

What are the different RNA interference methods?

I would like to know more on their advantages, disadvantage and their use in gene expression analysis.

10 November 2013 7,372 0 View

What are the best methods to evaluate and compare the gene expression in plants?

I want to check the gene expressions of two family of plants. What are the methods that I can use? I also want to know the limitations. A discussion on this topic is expected.

10 November 2013 2,508 3 View

What are plantibodies?

How are they different from normal antibodies? Seeking information about their generation and isolation.

09 October 2013 1,323 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?

31 July 2024 8,939 5 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Vincent Mulholland

Is this the one?

http://www.ncbi.nlm.nih.gov/nuccore/122703318

Nikhil Job

Yes, but sequence is not available.

Thank you,

Nikhil, scroll down the page - it's all there. Here a link to the FastA version:

http://www.ncbi.nlm.nih.gov/nuccore/122703318?report=fasta

thank you,

our sever was facing some problem with the link. now it is fine. thank you very much