Doctor Muhandas

10 Questions 30 Answers 0 Followers

Questions related from Doctor Muhandas

Can we dissolve plant proteins into human serum?

I want to check the presence of some specific antigenic proteins in a leaf. The system we have is used for detection of proteins using ELISA from serum samples. Is it possible that i crush my leaf...

04 April 2014 827 0 View

Can I use my plasmid as a positive control for ELISA?

I want to perform analysis on some plant samples for the detection of recombinant protein expression. For this purpose I want to use ELISA. Unfortunately I don't have a positive control. Is it...

03 March 2013 7,871 1 View

Storage of leaf sample for recombinant protein

I am working on plant transformation. I want to perform ELISA for my transformed plants and I need to transport my leaf sample to another place. What is the best way to transport the leaf samples...

08 August 2012 3,643 2 View

Multiple bands after PCR using nptII primer.

I am trying to detect putative transformed tomato plants by PCR using Kanamycin specific primer. Instead of getting one band of 720bp( primer designed for) I am getting multiple bands. I optimized...

08 August 2012 1,397 10 View

Southern blotting produced only a strong signal in the control but nothing in the test DNA. Did my transformation fail?

I transformed some plants, grown from initial stages till rooting on 100- 50 mg kanamycin media. After extracting DNA I did PCR using Gene specific primers and got clear sharp bands of desired...

06 June 2012 9,325 5 View

Labeling of southern probe by DIG PCR.

I am trying to label DNA probe by DIG PCR. First I successfully optimized the amplification of gene by simple PCR reagents. Then I used DIG components for PCR reaction mix as recommended by Roche...

05 May 2012 6,247 6 View

Purification of PCR product

Hello All, Can anyone have an experience to purify PCR product without using KITs suitable for ligation and cloning. If someone know any manual procedure please guide me about it. Thanks

02 February 2012 5,312 12 View

PCR product purification from gel.

Is there some easy manual( non kit) method to purify band from gel. Other than dialysis membrane and column elution. Thanks

02 February 2012 5,671 3 View

Problem in PCR

I designed some primers to amplify a tomato gene. When checked two primer pairs worked very well and gave me required length bands. I repeated this experiment three times and always get bands of...

02 February 2012 1,322 13 View

My question is that is there any way /method to check the function or activity of fruit specific promoter prior to transformation in plant?

I have isolated some fruit specific promoters by PCR amplification. On gel they are giving expected band size. I want to develop a gene cassette in which gene of interest will be under the control...

01 January 2012 1,714 6 View