Protein concentration in EMSA reaction?

11 December 2016 5,453 3 View

Sequences of pGWB3 vector with GUS reporter?

Can any one provide me the complete sequence of pGWB3 plant expression vector?

04 May 2015 10,000 4 View

Why does reaction volume decreases after restriction digestion of plasmid?

07 August 2014 8,250 4 View

How to design Real time PCR primers?

06 July 2014 3,330 8 View

How can I activate my oncogene of interest in plants?

I want to silence an oncogene (which is transfected) for a period of time and activate it. How can I do that? Could you suggest some papers for the above case,if any? This is a general case.

10 November 2013 3,699 1 View

What kind of expression and purification system can be used?

My protein of interest (human) contains glycosylation and having 34 kDa molecular weight. For crystallization purpose.

10 November 2013 2,911 1 View

Does anybody know the plant hormones which are secreted at the time of fruit formation in plants?

I am specifically interested in plant hormones which are either only secreted at the time of fruit formation or which are down regulated at that time. No need of specificity, can be any plant.

10 November 2013 9,946 12 View

What are the different RNA interference methods?

I would like to know more on their advantages, disadvantage and their use in gene expression analysis.

10 November 2013 7,290 0 View

What are the best methods to evaluate and compare the gene expression in plants?

I want to check the gene expressions of two family of plants. What are the methods that I can use? I also want to know the limitations. A discussion on this topic is expected.

10 November 2013 2,434 3 View

What are plantibodies?

How are they different from normal antibodies? Seeking information about their generation and isolation.

09 October 2013 1,243 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View