In an article I was reading it was mentioned that "Plasmid pGM-T (Tiangen Biotech) was used as the cloning vector" and "pET28a (+) was used as expression plasmid.
What is the difference between the two?
A cloning vector is a DNA molecule which can replicate in a suitable host organism, and into which a fragment of foreign DNA can be introduced. Most vectors used in molecular biology are based on bacterial plasmids and Bacteriophages (bacteria-infecting viruses). On the other hand expression vectors are vector that, in addition to the properties of a cloning vector, contains sequences that direct expression of the inserted DNA sequence in an appropriate host organism. It is also known as an expression construct, a plasmid or virus designed for protein expression in cells.
You can read this:
https://www.researchgate.net/post/Cloning_vector_and_Expression_vector
An expression vector is a plasmid designed for protein expression in various cells, but a cloning vector is a plasmid that can be stably maintained a foreign DNA fragment in an organism, and used for cloning purposes.
As suggested by the answers above, cloning and expression vectors have been differently designed for best performance in either cloning or protein expression, respectively. The most important aspect to remember is that an expression vector should NEVER be used to store DNA, because it will not be stable over time, and mutations are likely to occur. On the contrary, in principle you could exceptionally use a cloning vector to express a protein, although this is generally not done because you will likely get low yields etc.
The major difference between cloning vector and expression vector is in most of the cases cloning vector is high in copy number. Whereas, expression vectors are having tight regulatory sequences like ribosomal binding site (useful for ribosome binding), operator, etc. these helps in high level expression of your desired protein.
A cloning vector is a DNA molecule which can replicate in a suitable host organism, and into which a fragment of foreign DNA can be introduced. Most vectors used in molecular biology are based on bacterial plasmids and Bacteriophages (bacteria-infecting viruses). On the other hand expression vectors are vector that, in addition to the properties of a cloning vector, contains sequences that direct expression of the inserted DNA sequence in an appropriate host organism. It is also known as an expression construct, a plasmid or virus designed for protein expression in cells.
The answer is eloquently explained here http://www.majordifferences.com/2013/12/difference-between-cloning-vector-and.html
A cloning vector is simply a DNA molecule used to introduce a specific gene of interst into a target cell. It should have the basic constituents of any vector, mostly plasmids, icluding an origin of replication, specific to the cell in which it has to replicate, a selectable marker and multiple cloning site (MCS) for gene insertion.
An expression vector or expression construct is a vector used for protein expression in which a specific gene is introduced into a cell, and then the cell is being ordered, mostly with an inducer, to produce the protein encoded by that gene. Thus, it should contain the same previous basic constituents of any vector in addition to expression elements such as a promotor, a transcription termination sequence, a translation intitation sequence and a termination code. If you are going to purify your protein later, so it should also contain some protein tags such as (His) tag or other fusion proteins in order to facilitate the separation of your specific protein from the whole proteins that will be produced by the target cell.
Cloning vectors use for only cloning a gene or DNA fragment and not necessarily have protein expression elements. in contrast, a expression vector like pCAMBIA have larger size compare to a simple cloning vector like pGEM, and have expression elements like promoter, RBS, Terminator.
for more information please read my expression and cloning articles:
Article Isolation and bioinformatics study of TbJAMYC transcription ...
Article Constructing and transient expression of a gene cassette con...
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