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When I plate Mach 1 cells transformed with plasmid containing no insert on LB plates+ Kanamycin I see very little number to no colonies growing. But when an insert is present in the plasmid, the...
04 May 2015 3,358 3 View
Hello I was watching an introductory video to two-photon microscopy and came across the concepts of Rayleigh scattering and absorption. As I did not understand these concepts entirely, I would...
08 September 2014 3,371 4 View
I have some long non coding RNA sequences for which I would like to predict whether there any sites for the binding to specific miRNAs. I already tried using RNA hybrid...
04 May 2014 4,490 8 View
I would like to figure out the sequence conservation of some lncRNAs in different organisms (mouse, rat and human). I have already obtained the nucleotide sequence of the lncRNA gene (both introns...
04 May 2014 5,175 0 View
I am currently plotting some graphs on excel. I would like compare the expression of a certain gene in different conditions. I made my calculations on excel and ended up with a normal column...
03 April 2014 2,746 24 View
I am currently doing research on the bacteriocins Epidermicin, Nisin A, Subtilin and Sakacin A which are respectively naturally produced by by Staphylococcus epidermidis strain 224, Lactococcus...
31 December 2013 4,114 7 View
In an article I was reading it was mentioned that "Plasmid pGM-T (Tiangen Biotech) was used as the cloning vector" and "pET28a (+) was used as expression plasmid. What is the difference between...
31 December 2013 3,844 9 View
According to this article, "Angiogenesis is stimulated when tumor tissues require nutrients and oxygen" (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1993983/). But according to the Molecular Cell...
11 December 2013 4,091 5 View
From this website I managed to find the name of the genes that encode the bacteriocins that I am interested in (http://bactibase.pfba-lab-tun.org/main.php). Where can I find the gene sequence...
10 November 2013 7,690 4 View
Having a capsule is definitely advantageous for a bacteria, since the capsule is in most cases poorly anitphacytic and antigenic. So I am wondering: how is it even possible that some bacteria just...
10 November 2013 5,295 17 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
