Why E. coli transformed with plasmid containing no insert show a very small to no transformation efficiency compared to when an insert is present?

04 May 2015 3,269 3 View

Why does long wavelength light (i.e. red and near infrared) minimize absorption and scattering by biomolecules compared to short wavelength light?

Hello I was watching an introductory video to two-photon microscopy and came across the concepts of Rayleigh scattering and absorption. As I did not understand these concepts entirely, I would...

08 September 2014 3,259 4 View

How can I predict microRNAs binding sites on long non coding RNA sequence?

I have some long non coding RNA sequences for which I would like to predict whether there any sites for the binding to specific miRNAs. I already tried using RNA hybrid...

04 May 2014 4,437 8 View

How to make a long non coding RNA sequence conservation picture in different organism?

I would like to figure out the sequence conservation of some lncRNAs in different organisms (mouse, rat and human). I have already obtained the nucleotide sequence of the lncRNA gene (both introns...

04 May 2014 5,115 0 View

How can I insert statistical significance (i.e. t test P value < 0.05) annotations on top of my column bars on excel?

I am currently plotting some graphs on excel. I would like compare the expression of a certain gene in different conditions. I made my calculations on excel and ended up with a normal column...

03 April 2014 2,690 24 View

Can somebody provide me with a simple method to extract bacteriocins from natural bacterial producers grown in LB or agar plates?

I am currently doing research on the bacteriocins Epidermicin, Nisin A, Subtilin and Sakacin A which are respectively naturally produced by by Staphylococcus epidermidis strain 224, Lactococcus...

31 December 2013 4,069 7 View

What is the difference between a cloning vector and an expression plasmid?

In an article I was reading it was mentioned that "Plasmid pGM-T (Tiangen Biotech) was used as the cloning vector" and "pET28a (+) was used as expression plasmid. What is the difference between...

31 December 2013 3,782 9 View

Why do cancer cells stimulate vessels formation (angiogenesis) even if they are known to constitutively perform glycolysis regardless of O2 presence?

According to this article, "Angiogenesis is stimulated when tumor tissues require nutrients and oxygen" (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1993983/). But according to the Molecular Cell...

11 December 2013 4,035 5 View

Do all bacteria have a capsule? Do you know any examples of bacteria who do not have a capsule?

Having a capsule is definitely advantageous for a bacteria, since the capsule is in most cases poorly anitphacytic and antigenic. So I am wondering: how is it even possible that some bacteria just...

10 November 2013 5,240 17 View

Can anyone reccommend an online databse/website where I can find gene sequences of bacteriocins?

From this website I managed to find the name of the genes that encode the bacteriocins that I am interested in (http://bactibase.pfba-lab-tun.org/main.php). Where can I find the gene sequence...

10 November 2013 7,640 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View