Why E. coli transformed with plasmid containing no insert show a very small to no transformation efficiency compared to when an insert is present?

04 May 2015 3,269 3 View

Why does long wavelength light (i.e. red and near infrared) minimize absorption and scattering by biomolecules compared to short wavelength light?

Hello I was watching an introductory video to two-photon microscopy and came across the concepts of Rayleigh scattering and absorption. As I did not understand these concepts entirely, I would...

08 September 2014 3,259 4 View

How can I predict microRNAs binding sites on long non coding RNA sequence?

I have some long non coding RNA sequences for which I would like to predict whether there any sites for the binding to specific miRNAs. I already tried using RNA hybrid...

04 May 2014 4,437 8 View

How to make a long non coding RNA sequence conservation picture in different organism?

I would like to figure out the sequence conservation of some lncRNAs in different organisms (mouse, rat and human). I have already obtained the nucleotide sequence of the lncRNA gene (both introns...

04 May 2014 5,115 0 View

How can I insert statistical significance (i.e. t test P value < 0.05) annotations on top of my column bars on excel?

I am currently plotting some graphs on excel. I would like compare the expression of a certain gene in different conditions. I made my calculations on excel and ended up with a normal column...

03 April 2014 2,690 24 View

Can somebody provide me with a simple method to extract bacteriocins from natural bacterial producers grown in LB or agar plates?

I am currently doing research on the bacteriocins Epidermicin, Nisin A, Subtilin and Sakacin A which are respectively naturally produced by by Staphylococcus epidermidis strain 224, Lactococcus...

31 December 2013 4,069 7 View

What is the difference between a cloning vector and an expression plasmid?

In an article I was reading it was mentioned that "Plasmid pGM-T (Tiangen Biotech) was used as the cloning vector" and "pET28a (+) was used as expression plasmid. What is the difference between...

31 December 2013 3,782 9 View

What exactly is a ribozyme and can you explain in general terms how I can design one that will prevent expression of specific proteins?

Question above

11 December 2013 5,115 0 View

Why do cancer cells stimulate vessels formation (angiogenesis) even if they are known to constitutively perform glycolysis regardless of O2 presence?

According to this article, "Angiogenesis is stimulated when tumor tissues require nutrients and oxygen" (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1993983/). But according to the Molecular Cell...

11 December 2013 4,035 5 View

Do all bacteria have a capsule? Do you know any examples of bacteria who do not have a capsule?

Having a capsule is definitely advantageous for a bacteria, since the capsule is in most cases poorly anitphacytic and antigenic. So I am wondering: how is it even possible that some bacteria just...

10 November 2013 5,240 17 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View