Parisa Farrokh

6 Questions 20 Answers 0 Followers

Questions related from Parisa Farrokh

Is it possible to limit the result of BLAST to transcripts of the special tissue?

"bioinformatics" , "real-time PCR" , "primer design"

01 January 2015 1,668 3 View

Can anyone help me with interpreting the results of a protein sequence analyzed with Delta-blast and blast-p?

I used blastp and DELTA-BLAST algorithms for the same protein sequence, but I am confused with the results. Putative conserved domains have been detected by blastp, but nothing has been detected...

04 April 2014 7,810 2 View

Can anyone explain why in the initial denaturation of the TAIL-PCR most of the time we use two temperatures (for example 93 and 95 °C)?

Is it possible to use one temperature like usual PCR (95 °C for 5 min)?

10 October 2013 7,225 1 View

The order of loop refinement in protein modeling.

I am working with modeller and I would like to know the order of loop refinement (for example starting with loop3, then loop1 and then loop2) has any effects on the final model?

04 April 2013 3,443 1 View

How to determine the best simulation time for molecular dynamic in GROMACS?

I made a model for my protein using Modeller with template having 46% identity. Now I want to use Gromacs for energy minimization and molecular dynamics simulation. I see different simulation time...

02 February 2013 2,216 2 View

Why a mutant with single amino acid mutation dose not express in E.coli (BL 21 pLysS strain)?

I have a problem with expression of a mutant protein in E.coli (BL 21 pLysS strain). Recently I could expressed the native and two other mutants from this protein in the same host by pET26. Only...

11 November 2012 9,199 7 View