Paolo Marangio

13 Questions 4 Answers 0 Followers

Questions related from Paolo Marangio

Why E. coli transformed with plasmid containing no insert show a very small to no transformation efficiency compared to when an insert is present?

When I plate Mach 1 cells transformed with plasmid containing no insert on LB plates+ Kanamycin I see very little number to no colonies growing. But when an insert is present in the plasmid, the...

05 May 2015 3,326 3 View

Why does long wavelength light (i.e. red and near infrared) minimize absorption and scattering by biomolecules compared to short wavelength light?

Hello I was watching an introductory video to two-photon microscopy and came across the concepts of Rayleigh scattering and absorption. As I did not understand these concepts entirely, I would...

09 September 2014 3,331 4 View

How to make a long non coding RNA sequence conservation picture in different organism?

I would like to figure out the sequence conservation of some lncRNAs in different organisms (mouse, rat and human). I have already obtained the nucleotide sequence of the lncRNA gene (both introns...

05 May 2014 5,146 0 View

How can I predict microRNAs binding sites on long non coding RNA sequence?

I have some long non coding RNA sequences for which I would like to predict whether there any sites for the binding to specific miRNAs. I already tried using RNA hybrid...

05 May 2014 4,465 8 View

How can I insert statistical significance (i.e. t test P value < 0.05) annotations on top of my column bars on excel?

I am currently plotting some graphs on excel. I would like compare the expression of a certain gene in different conditions. I made my calculations on excel and ended up with a normal column...

04 April 2014 2,724 24 View

What is the difference between a cloning vector and an expression plasmid?

In an article I was reading it was mentioned that "Plasmid pGM-T (Tiangen Biotech) was used as the cloning vector" and "pET28a (+) was used as expression plasmid. What is the difference between...

01 January 2014 3,819 9 View

Can somebody provide me with a simple method to extract bacteriocins from natural bacterial producers grown in LB or agar plates?

I am currently doing research on the bacteriocins Epidermicin, Nisin A, Subtilin and Sakacin A which are respectively naturally produced by by Staphylococcus epidermidis strain 224, Lactococcus...

01 January 2014 4,095 7 View

What exactly is a ribozyme and can you explain in general terms how I can design one that will prevent expression of specific proteins?

Question above

12 December 2013 5,149 0 View

Why do cancer cells stimulate vessels formation (angiogenesis) even if they are known to constitutively perform glycolysis regardless of O2 presence?

According to this article, "Angiogenesis is stimulated when tumor tissues require nutrients and oxygen" (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1993983/). But according to the Molecular Cell...

12 December 2013 4,067 5 View

How are plasmid containing antibiotic resistant genes able to promote themselves so that they are passed on to other bacteria?

I am interested in how this actually works. Is the bacterium sort of "aware" of the fact that its plasmid contains antibiotic resistance gene and therefore passes the plasmid to the other...

11 November 2013 641 3 View

Do all bacteria have a capsule? Do you know any examples of bacteria who do not have a capsule?

Having a capsule is definitely advantageous for a bacteria, since the capsule is in most cases poorly anitphacytic and antigenic. So I am wondering: how is it even possible that some bacteria just...

11 November 2013 5,274 17 View

Can anyone reccommend an online databse/website where I can find gene sequences of bacteriocins?

From this website I managed to find the name of the genes that encode the bacteriocins that I am interested in (http://bactibase.pfba-lab-tun.org/main.php). Where can I find the gene sequence...

11 November 2013 7,670 4 View

Which substance when taken in by a human individual it causes a harmless change in expression of one or more genes within a week?

So i want to do a 4 weeks study in which I want to investigate a change in gene expression as caused by intake of a substance. I am planning to do this on humans and by substance i mean nutrients,...

07 July 2013 8,481 0 View