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Questions related from Paolo Marangio
When I plate Mach 1 cells transformed with plasmid containing no insert on LB plates+ Kanamycin I see very little number to no colonies growing. But when an insert is present in the plasmid, the...
05 May 2015 3,337 3 View
Hello I was watching an introductory video to two-photon microscopy and came across the concepts of Rayleigh scattering and absorption. As I did not understand these concepts entirely, I would...
09 September 2014 3,348 4 View
I would like to figure out the sequence conservation of some lncRNAs in different organisms (mouse, rat and human). I have already obtained the nucleotide sequence of the lncRNA gene (both introns...
05 May 2014 5,152 0 View
I have some long non coding RNA sequences for which I would like to predict whether there any sites for the binding to specific miRNAs. I already tried using RNA hybrid...
05 May 2014 4,471 8 View
I am currently plotting some graphs on excel. I would like compare the expression of a certain gene in different conditions. I made my calculations on excel and ended up with a normal column...
04 April 2014 2,730 24 View
In an article I was reading it was mentioned that "Plasmid pGM-T (Tiangen Biotech) was used as the cloning vector" and "pET28a (+) was used as expression plasmid. What is the difference between...
01 January 2014 3,826 9 View
I am currently doing research on the bacteriocins Epidermicin, Nisin A, Subtilin and Sakacin A which are respectively naturally produced by by Staphylococcus epidermidis strain 224, Lactococcus...
01 January 2014 4,100 7 View
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12 December 2013 5,156 0 View
According to this article, "Angiogenesis is stimulated when tumor tissues require nutrients and oxygen" (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1993983/). But according to the Molecular Cell...
12 December 2013 4,072 5 View
I am interested in how this actually works. Is the bacterium sort of "aware" of the fact that its plasmid contains antibiotic resistance gene and therefore passes the plasmid to the other...
11 November 2013 645 3 View
Having a capsule is definitely advantageous for a bacteria, since the capsule is in most cases poorly anitphacytic and antigenic. So I am wondering: how is it even possible that some bacteria just...
11 November 2013 5,279 17 View
From this website I managed to find the name of the genes that encode the bacteriocins that I am interested in (http://bactibase.pfba-lab-tun.org/main.php). Where can I find the gene sequence...
11 November 2013 7,675 4 View
So i want to do a 4 weeks study in which I want to investigate a change in gene expression as caused by intake of a substance. I am planning to do this on humans and by substance i mean nutrients,...
07 July 2013 8,486 0 View
