What is the correct method to calculate the IC50 value for the MTT assay?

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Taufeeque Ali

GraphPad Prism with the four-parameter logistic (4PL) model is generally considered the best and most suitable method for calculating IC50 values from MTT assays. This model accommodates the complexities of biological data, providing a more accurate and reliable result.

Karthick Mk

Taufeeque Ali Thank you for the valuable information, sir.