Karthick Mk

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Questions related from Karthick Mk

How to interpret the reduced glutathione (GSH) assay?

I had done the Reduced Glutathione assay in rat liver tissue (10% homogenate in PO4 buffer, pH 7.4) using Beutler et al., 1963 by DTNB method. The results are comes around more than 300 units. But...

13 March 2025 4,750 0 View

How to get rid of "Yellow background indicates a high reference wavelength read" in microplate reader?

I had experienced the "Yellow background indicates a high reference wavelength read", while quantifying RNA samples (Trizol kit). Rather than DNA and cDNA samples were quantified well in the same...

29 October 2024 7,662 5 View

What is error occurs in lipid peroxidation assay using toxicity-induced liver samples (1%, PO4 buffer)?

I had also experienced the no colour change problem using 1% liver homogenate for the lipid peroxidation assay. I had a doubt which is whether the n-butanol : pyridine mixture is required for the...

21 August 2024 7,452 4 View

What is the correct method to calculate the IC50 value for the MTT assay?

I had done calculating MTT assay using Slope intercept method - Excel Four parameter logistic regression model-web portal Using Graphpad prism. Among them which one is the best and suitable for...

02 June 2024 2,654 3 View

What is the correct method to calculate the IC50 value for the MTT assay?

I had done calculating MTT assay using Slope intercept method - Excel Four parameter logistic regression model-web portal Using Graphpad prism. Among them which one is the best and suitable for...

01 January 1970 5,729 2 View