Taufeeque Ali

5 Questions 35 Answers 0 Followers

Questions related from Taufeeque Ali

What experiments would you recommend for evaluating drug safety, particularly in terms of histopathological changes, and molecular alterations?

I am currently investigating the safety profile of a drug in nude mice models, focusing on organs and extracted tumors. I have utilized H&E staining and IHC analysis in my experiments....

02 February 2024 1,219 1 View

Is there a more efficient method for neutralizing intracellular H2O2 compared to the conventional and costly PEG-catalase?

Our prodrug is activated through ROS-mediated reactions, particularly with H2O2, as indicated by our in vitro data. I'm currently working on designing an experiment to effectively reduce...

07 September 2023 7,654 0 View

Has anyone observed an "ABNORMAL" proliferation of the U87 Glioblastoma cells in comparison to your previous cultures?

I have been using U87 cells for over a year. I study effects of anti-cancer drugs on several cell line including U87. Over the past few months, the cultures are 100% confluent on the second day...

13 April 2023 2,481 2 View

Will an Epifluorescence microscope with an RFP cube work for Ex/Em = 543 nm/545-750 nm?

I am planning to stain cells with an Orange dye to measure intracellular hydrogen peroxide. Link for the dye kit is below. Protocol suggest examining cells at Ex/Em = 543 nm/545-750 nm. I want to...

03 April 2023 7,884 0 View

In vivo measurement of Hydrogen Peroxide?

I am planning to run some experiments to measure hydrogen peroxide in vivo. I have come across some methods such as use of Hyper and micro-dialysis method. Is there a simpler way to measure...

16 September 2022 8,695 0 View