Hello Sara Haji Rasul

I would recommend cyQUANT from Thermo Fischer because this assay is quick to perform with minimal hands-on time. Moreover, this method offers improved accuracy over metabolically based cell proliferation or cytotoxicity assays that can be influenced by cell changes that are unrelated to cell number.

The main component of cyQUANT cell proliferation assay kit is a proprietary dye that exhibits strong fluorescence enhancement when bound to nucleic acids. With this dye, you can measure a sample's DNA content and compare it to standards, directly quantifying the entire cell population within a broad linear detection range.

Since the cellular DNA content is highly regulated, it is closely proportional to cell number. Changes in nucleic acid content can serve as a sensitive indicator of overall cell proliferation, as well as cytotoxic events that may affect cell proliferation.

So, I suggest that you go for cyQUANT cell proliferation assay kit.

Best.

I will recommend Real-time Glo from Promega, which works by generating a luminescent signal dependent on continuous reduction of the MT cell viability substrate by viable cells and rapid turnover by NanoLuc® Luciferase. Apart from the fact that it is a straightforward assay. It can be used to assay proliferation rate for 72 hrs.