Does Salmonella Typhimurieum cause latent infection?

I want to know whether Salmonella Typhimurium cause latent infection in mice..

04 May 2018 7,427 0 View

How to calculate in vitro and invivo mutation frequency or rate?

Hello everyone, I want to infect the macrophages with Bacteria and after particular treatment I want to know the damage or mutation of bacterial DNA (Intracellular bacterial).I have seen some...

03 April 2017 7,617 0 View

How to Qantify intracellular Pathogen's DNA damage ?

Hi, every one.. I wasn't to quantify the DNA damage of the macrophage infected intracellular pathogen's DNA damage. is there any methods

02 March 2017 6,660 7 View

How to assess the intracellular parasite killing?

10 November 2016 9,667 4 View

How to write a review paper?

what are the things one should keep in mind when writing a review paper?

10 November 2016 9,022 0 View

Can I use PCR product as template for in vitro translation?

I have Promega TNT T7 insect protein expression system..can I use PCR product as a template for in vitro protein synthesis ? if yes should I need to add any extra sequences to the primers such as...

04 May 2016 2,985 2 View

I want to study the migration of neutrophils. Can any one tell me how to do it?

I have cell culture insert plate of 3.o um and 8.0 um , which one better to study neutrophil migration?

31 December 2015 454 1 View

Peptide stabilization assay?

31 December 2015 9,624 8 View

Can anyone tell me, what is the solubility of amonium tetrathiomolybdate?

Amonium tetrathiomolybdate sigma (cat, no 323446)

06 July 2014 9,548 0 View

How do I prepare a stock solution and stain macrophage?

Macrophages staining. I have Giemsa powder (Sigma G5637).

02 March 2014 5,480 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View