Amonium tetrathiomolybdate sigma (cat, no 323446)
I want to know whether Salmonella Typhimurium cause latent infection in mice..
04 May 2018 7,549 0 View
Hello everyone, I want to infect the macrophages with Bacteria and after particular treatment I want to know the damage or mutation of bacterial DNA (Intracellular bacterial).I have seen some...
03 April 2017 7,723 0 View
Hi, every one.. I wasn't to quantify the DNA damage of the macrophage infected intracellular pathogen's DNA damage. is there any methods
02 March 2017 6,773 7 View
I want assess the rate of intracellular parasite killing in side the infected macrophages in in vitro experiment. what method is opt for assess live or dead parasites inside the macrophages?...
10 November 2016 9,780 4 View
what are the things one should keep in mind when writing a review paper?
10 November 2016 9,119 0 View
I am working on the diagnosis of Visceral leishmaniasis based on the urine DNA. i want to know the average quantity of DNA in urine of visceral leishmaniasis patients. anyone has any idea about...
09 October 2016 6,970 3 View
I have Promega TNT T7 insect protein expression system..can I use PCR product as a template for in vitro protein synthesis ? if yes should I need to add any extra sequences to the primers such as...
04 May 2016 3,096 2 View
I have cell culture insert plate of 3.o um and 8.0 um , which one better to study neutrophil migration?
31 December 2015 573 1 View
some people are stimulating T2 cells with peptide for 18 hrs and some people use Beta 2-macroglobuline and some protocol says incubate peptide with T2 cells for 18 hrs and then incubated...
31 December 2015 9,821 8 View
Macrophages staining. I have Giemsa powder (Sigma G5637).
02 March 2014 5,594 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
