Does Salmonella Typhimurieum cause latent infection?

I want to know whether Salmonella Typhimurium cause latent infection in mice..

04 May 2018 7,427 0 View

How to calculate in vitro and invivo mutation frequency or rate?

Hello everyone, I want to infect the macrophages with Bacteria and after particular treatment I want to know the damage or mutation of bacterial DNA (Intracellular bacterial).I have seen some...

03 April 2017 7,617 0 View

How to Qantify intracellular Pathogen's DNA damage ?

Hi, every one.. I wasn't to quantify the DNA damage of the macrophage infected intracellular pathogen's DNA damage. is there any methods

02 March 2017 6,660 7 View

How to assess the intracellular parasite killing?

10 November 2016 9,667 4 View

How to write a review paper?

what are the things one should keep in mind when writing a review paper?

10 November 2016 9,022 0 View

What is the concentration of DNA in urine?

I am working on the diagnosis of Visceral leishmaniasis based on the urine DNA. i want to know the average quantity of DNA in urine of visceral leishmaniasis patients. anyone has any idea about...

09 October 2016 6,859 3 View

Can I use PCR product as template for in vitro translation?

I have Promega TNT T7 insect protein expression system..can I use PCR product as a template for in vitro protein synthesis ? if yes should I need to add any extra sequences to the primers such as...

04 May 2016 2,985 2 View

I want to study the migration of neutrophils. Can any one tell me how to do it?

I have cell culture insert plate of 3.o um and 8.0 um , which one better to study neutrophil migration?

31 December 2015 454 1 View

Peptide stabilization assay?

31 December 2015 9,624 8 View

How do I prepare a stock solution and stain macrophage?

Macrophages staining. I have Giemsa powder (Sigma G5637).

02 March 2014 5,480 0 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View