Does Salmonella Typhimurieum cause latent infection?

I want to know whether Salmonella Typhimurium cause latent infection in mice..

04 May 2018 7,427 0 View

How to Qantify intracellular Pathogen's DNA damage ?

Hi, every one.. I wasn't to quantify the DNA damage of the macrophage infected intracellular pathogen's DNA damage. is there any methods

02 March 2017 6,660 7 View

How to assess the intracellular parasite killing?

10 November 2016 9,667 4 View

How to write a review paper?

what are the things one should keep in mind when writing a review paper?

10 November 2016 9,022 0 View

What is the concentration of DNA in urine?

I am working on the diagnosis of Visceral leishmaniasis based on the urine DNA. i want to know the average quantity of DNA in urine of visceral leishmaniasis patients. anyone has any idea about...

09 October 2016 6,859 3 View

Can I use PCR product as template for in vitro translation?

I have Promega TNT T7 insect protein expression system..can I use PCR product as a template for in vitro protein synthesis ? if yes should I need to add any extra sequences to the primers such as...

04 May 2016 2,985 2 View

I want to study the migration of neutrophils. Can any one tell me how to do it?

I have cell culture insert plate of 3.o um and 8.0 um , which one better to study neutrophil migration?

31 December 2015 454 1 View

Peptide stabilization assay?

31 December 2015 9,624 8 View

Can anyone tell me, what is the solubility of amonium tetrathiomolybdate?

Amonium tetrathiomolybdate sigma (cat, no 323446)

06 July 2014 9,548 0 View

How do I prepare a stock solution and stain macrophage?

Macrophages staining. I have Giemsa powder (Sigma G5637).

02 March 2014 5,480 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View