Macrophages staining. I have Giemsa powder (Sigma G5637).
I want to know whether Salmonella Typhimurium cause latent infection in mice..
04 May 2018 7,549 0 View
Hello everyone, I want to infect the macrophages with Bacteria and after particular treatment I want to know the damage or mutation of bacterial DNA (Intracellular bacterial).I have seen some...
03 April 2017 7,723 0 View
Hi, every one.. I wasn't to quantify the DNA damage of the macrophage infected intracellular pathogen's DNA damage. is there any methods
02 March 2017 6,773 7 View
I want assess the rate of intracellular parasite killing in side the infected macrophages in in vitro experiment. what method is opt for assess live or dead parasites inside the macrophages?...
10 November 2016 9,780 4 View
what are the things one should keep in mind when writing a review paper?
10 November 2016 9,119 0 View
I am working on the diagnosis of Visceral leishmaniasis based on the urine DNA. i want to know the average quantity of DNA in urine of visceral leishmaniasis patients. anyone has any idea about...
09 October 2016 6,970 3 View
I have Promega TNT T7 insect protein expression system..can I use PCR product as a template for in vitro protein synthesis ? if yes should I need to add any extra sequences to the primers such as...
04 May 2016 3,096 2 View
I have cell culture insert plate of 3.o um and 8.0 um , which one better to study neutrophil migration?
31 December 2015 573 1 View
some people are stimulating T2 cells with peptide for 18 hrs and some people use Beta 2-macroglobuline and some protocol says incubate peptide with T2 cells for 18 hrs and then incubated...
31 December 2015 9,821 8 View
Amonium tetrathiomolybdate sigma (cat, no 323446)
06 July 2014 9,672 0 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...
01 August 2024 476 2 View
I have a question about human macrophages cell surface markers.I want to stain the unpolarized human macrophages for flow cytometric sorting without permeabilizing them. It is because I need to...
29 July 2024 236 2 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...
15 July 2024 5,153 2 View
Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...
15 July 2024 4,615 1 View
Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...
03 July 2024 3,884 5 View