I want to know whether Salmonella Typhimurium cause latent infection in mice..
Hello everyone, I want to infect the macrophages with Bacteria and after particular treatment I want to know the damage or mutation of bacterial DNA (Intracellular bacterial).I have seen some...
03 April 2017 7,723 0 View
Hi, every one.. I wasn't to quantify the DNA damage of the macrophage infected intracellular pathogen's DNA damage. is there any methods
02 March 2017 6,773 7 View
I want assess the rate of intracellular parasite killing in side the infected macrophages in in vitro experiment. what method is opt for assess live or dead parasites inside the macrophages?...
10 November 2016 9,780 4 View
what are the things one should keep in mind when writing a review paper?
10 November 2016 9,119 0 View
I am working on the diagnosis of Visceral leishmaniasis based on the urine DNA. i want to know the average quantity of DNA in urine of visceral leishmaniasis patients. anyone has any idea about...
09 October 2016 6,970 3 View
I have Promega TNT T7 insect protein expression system..can I use PCR product as a template for in vitro protein synthesis ? if yes should I need to add any extra sequences to the primers such as...
04 May 2016 3,096 2 View
I have cell culture insert plate of 3.o um and 8.0 um , which one better to study neutrophil migration?
31 December 2015 573 1 View
some people are stimulating T2 cells with peptide for 18 hrs and some people use Beta 2-macroglobuline and some protocol says incubate peptide with T2 cells for 18 hrs and then incubated...
31 December 2015 9,821 8 View
Amonium tetrathiomolybdate sigma (cat, no 323446)
06 July 2014 9,672 0 View
Macrophages staining. I have Giemsa powder (Sigma G5637).
02 March 2014 5,594 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View