Can I use PCR product as template for in vitro translation?

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Dominique Liger

Hi there,

Yes you can provided your PCR product Template contains the sequences required for an efficient transcription/translation process in an eukaryotic context (promoter + untranslated region (UTR) sequences at the 5´ and 3´ ends of the gene coding region to enhance translation efficiency).

Vijayamahantesh Bajegoudar

Derar Dominique,

Thank you very much for your advice.. I have one more query. I read online that we need to add promoter sequence-spacer-Kozak sequence- gene...

5′-TAA TAC GAC TCA CTA TAG GG- -(N3-6)-CCACCATGG (N-17-22)...

My question is what should be the pacer sequence in between the promoter and Kozak sequence? Shall I add any sequence or is there any particular sequence ?

Please help me...