Should 5% Oil Red O in propylene glycol stock be stored at room temperature?

Dear RGs, I have prepared 0.5 % Oil Red O (SIGMA) in propylene glycol. Should I keep the stock at 4 degrees or at room temperature? Thanks a lot in advance. Best Regards, Matio

01 February 2019 6,645 2 View

Does anybody know how to prepare sugar-stock solution for cell culture experiment?

Hi. I would like to prepare stock solution for glucose or galactose or lactose for cell culture experiment. I will be very happy if you can give me tips, such as: in what (PBS, medium) to dissolve...

06 July 2018 5,314 1 View

Has anyone experienced with GLUCOSE tolerance test in neonatal mice pups (P0-P1)?

I would like to make the test in mouse pups (P0/P1). I thought to use 2 ways: via gavage and IPGTT. 1) Could you suggest me the size of the tube and of the syringe that should be used for the...

05 June 2018 7,689 0 View

Has anyone experienced with LACTOSE tolerance test with mice?

Dear RG-friends, I would like to do a lactose tolerance test in mouse newborns by measurement of the blood glucose level with a normal glucometer. I am wondering if someone did it and if kindly...

05 June 2018 7,136 2 View

Does anyone have experience with smooth muscle cell transfection?

Dear RGs, I would like to run transfection experiments by using rat primary SMCs and I am wondering if someone can give advices about it. Thank you so much in advance. Best Regards, Mario

10 November 2017 2,453 4 View

What is the best and easy and fast way to knockdown a gene in adult rat?

08 September 2017 261 0 View

Cerament and cell therapy ?

07 August 2017 8,866 0 View

Can Dot Blot be of help when Western Blot does not work for certain Abs?

It was not possible to see any expected band for the target, with a very low molecular weight (8-12 kD), by WB. The membrane was very clean after incubation with Ab-I-AbII. Probably we lost...

02 March 2016 7,273 4 View

Freezing and culturing Human T-cells?

Please, Could you tell me what is the best condition (cell density/media..)to freeze and to culture human T-cells? How long can T-cells be in culture? Thank you in advance Mario

01 February 2016 5,634 3 View

What does the ratio phospholamban/SERCA (at message level) mean?

31 December 2015 8,131 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to figure out easily the right journal with the right scope for your paper?

According to my experience most of the selected journals during paper submission are based on journal text matching suggested results from Springer, Elsevier,,,Etc. then the manuscript happened to...

01 March 2021 1,786 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View