Should 5% Oil Red O in propylene glycol stock be stored at room temperature?

More Mario Grossi's questions See All

Does anybody know how to prepare sugar-stock solution for cell culture experiment?

Hi. I would like to prepare stock solution for glucose or galactose or lactose for cell culture experiment. I will be very happy if you can give me tips, such as: in what (PBS, medium) to dissolve...

06 July 2018 5,400 1 View

Has anyone experienced with LACTOSE tolerance test with mice?

Dear RG-friends, I would like to do a lactose tolerance test in mouse newborns by measurement of the blood glucose level with a normal glucometer. I am wondering if someone did it and if kindly...

05 June 2018 7,229 2 View

Has anyone experienced with GLUCOSE tolerance test in neonatal mice pups (P0-P1)?

I would like to make the test in mouse pups (P0/P1). I thought to use 2 ways: via gavage and IPGTT. 1) Could you suggest me the size of the tube and of the syringe that should be used for the...

05 June 2018 7,770 0 View

What is the best way to isolate total RNA from plasma?

Dear RG-friends, I would like to isolate total RNA from plasma. Many thanks in advance for your timing and consideration. Best Regards, Mario

03 April 2018 1,753 2 View

Does anyone have experience with smooth muscle cell transfection?

Dear RGs, I would like to run transfection experiments by using rat primary SMCs and I am wondering if someone can give advices about it. Thank you so much in advance. Best Regards, Mario

10 November 2017 2,535 4 View

What is the best and easy and fast way to knockdown a gene in adult rat?

Dear RG-friends, I would like to knockdown a gene in adult rat and focusing first at a global level and then target a specific tissue/organ. Would you suggest me an alternative way to the...

08 September 2017 356 0 View

Cerament and cell therapy ?

Dear RG-friends, Would it be possible to use cerament for local cell therapy? I would be more than happy to know your opinion about it. Many thanks in advance. Best Regards, Mario 😉

07 August 2017 8,963 0 View

Can Dot Blot be of help when Western Blot does not work for certain Abs?

It was not possible to see any expected band for the target, with a very low molecular weight (8-12 kD), by WB. The membrane was very clean after incubation with Ab-I-AbII. Probably we lost...

02 March 2016 7,359 4 View

Freezing and culturing Human T-cells?

Please, Could you tell me what is the best condition (cell density/media..)to freeze and to culture human T-cells? How long can T-cells be in culture? Thank you in advance Mario

01 February 2016 5,771 3 View

What does the ratio phospholamban/SERCA (at message level) mean?

Several studies show the expression of SERCA and phospholamban at message levels. I have seen also that they have used the ratio of those genes. Could you help me to find the reason for it, please?

31 December 2015 8,266 2 View

Ethylene glycol is newtonian or non newtonian fluid?

Some sources say it is treated as Newtonian as ethylene glycol has a higher viscosity than water, which affects the flow characteristics in simulations. But some also say it is non-Newtonian.

09 August 2024 2,111 2 View

How to heat polymer for about 130° without oil bath ?

is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it

06 August 2024 947 1 View

Do you think can be any gas and oil bearing rocks in Eastern part of Iran?

I want to know more about petroleum deposits in Iran.

02 August 2024 8,725 3 View

Heavy Crude oil PVT Data?

Hello i need heavy oil PVT data having viscoisty of 2000-3000 cP. If someone have it or research papers about it, kindly share it with me Thanks

25 July 2024 6,940 1 View

What are the most PROBLEMS of HYBRID nanofluid in oil industry ?

explain your idea

23 July 2024 1,197 0 View

Do you think can be any gas and oil reservoirs in central of Iran?

I want to know more about gas and oil reservoirs in Iran.

14 July 2024 3,350 2 View

How am I supposed to find justifications for the unusual fatty acid profile in broiler diet?

I've obtained a fatty acid profile of broiler diet comprised of SFA 48 to 93%, MUFA (22 to 40%), PUFA (non determined to 10.4%. The PUFA composition is mainly n-6 and almost no value for n-3. The...

08 July 2024 9,165 4 View

Determination of fatty acids in fish?

I measured the fatty acids in the fish, using the internal standard method in the national standard method, I first extracted the fish oil with cable extraction, please ask me how many grams of...

02 July 2024 900 1 View

Is there a vegetation zone dividing location based on climate or geographic parameters available by province level?

I am researching the traceability of palm oil and need a parameter that can differentiate between provinces around the world. I want to group the provinces by their vegetation zone and see if it's...

01 July 2024 888 2 View

How to know if the oil palm tree is functioning properly in terms of absorption of different nutrient?

The idea is to design an IoT system that is able to capture real-time conditions from an oil palm tree to ensure optimal growth. Nutrients such as Sulfur, Nitrogen, Boron, Zinc, Copper and Iron.

30 June 2024 3,046 2 View