Should 5% Oil Red O in propylene glycol stock be stored at room temperature?

Dear RGs, I have prepared 0.5 % Oil Red O (SIGMA) in propylene glycol. Should I keep the stock at 4 degrees or at room temperature? Thanks a lot in advance. Best Regards, Matio

01 February 2019 6,645 2 View

Does anybody know how to prepare sugar-stock solution for cell culture experiment?

Hi. I would like to prepare stock solution for glucose or galactose or lactose for cell culture experiment. I will be very happy if you can give me tips, such as: in what (PBS, medium) to dissolve...

06 July 2018 5,314 1 View

Has anyone experienced with GLUCOSE tolerance test in neonatal mice pups (P0-P1)?

I would like to make the test in mouse pups (P0/P1). I thought to use 2 ways: via gavage and IPGTT. 1) Could you suggest me the size of the tube and of the syringe that should be used for the...

05 June 2018 7,689 0 View

What is the best way to isolate total RNA from plasma?

Dear RG-friends, I would like to isolate total RNA from plasma. Many thanks in advance for your timing and consideration. Best Regards, Mario

03 April 2018 1,662 2 View

Does anyone have experience with smooth muscle cell transfection?

Dear RGs, I would like to run transfection experiments by using rat primary SMCs and I am wondering if someone can give advices about it. Thank you so much in advance. Best Regards, Mario

10 November 2017 2,453 4 View

What is the best and easy and fast way to knockdown a gene in adult rat?

08 September 2017 261 0 View

Cerament and cell therapy ?

07 August 2017 8,866 0 View

Can Dot Blot be of help when Western Blot does not work for certain Abs?

It was not possible to see any expected band for the target, with a very low molecular weight (8-12 kD), by WB. The membrane was very clean after incubation with Ab-I-AbII. Probably we lost...

02 March 2016 7,273 4 View

Freezing and culturing Human T-cells?

Please, Could you tell me what is the best condition (cell density/media..)to freeze and to culture human T-cells? How long can T-cells be in culture? Thank you in advance Mario

01 February 2016 5,634 3 View

What does the ratio phospholamban/SERCA (at message level) mean?

31 December 2015 8,131 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View