Mario Grossi

11 Questions 27 Answers 0 Followers

Questions related from Mario Grossi

Should 5% Oil Red O in propylene glycol stock be stored at room temperature?

Dear RGs, I have prepared 0.5 % Oil Red O (SIGMA) in propylene glycol. Should I keep the stock at 4 degrees or at room temperature? Thanks a lot in advance. Best Regards, Matio

02 February 2019 6,706 2 View

Does anybody know how to prepare sugar-stock solution for cell culture experiment?

Hi. I would like to prepare stock solution for glucose or galactose or lactose for cell culture experiment. I will be very happy if you can give me tips, such as: in what (PBS, medium) to dissolve...

07 July 2018 5,360 1 View

Has anyone experienced with LACTOSE tolerance test with mice?

Dear RG-friends, I would like to do a lactose tolerance test in mouse newborns by measurement of the blood glucose level with a normal glucometer. I am wondering if someone did it and if kindly...

06 June 2018 7,186 2 View

Has anyone experienced with GLUCOSE tolerance test in neonatal mice pups (P0-P1)?

I would like to make the test in mouse pups (P0/P1). I thought to use 2 ways: via gavage and IPGTT. 1) Could you suggest me the size of the tube and of the syringe that should be used for the...

06 June 2018 7,740 0 View

What is the best way to isolate total RNA from plasma?

Dear RG-friends, I would like to isolate total RNA from plasma. Many thanks in advance for your timing and consideration. Best Regards, Mario

04 April 2018 1,712 2 View

Does anyone have experience with smooth muscle cell transfection?

Dear RGs, I would like to run transfection experiments by using rat primary SMCs and I am wondering if someone can give advices about it. Thank you so much in advance. Best Regards, Mario

11 November 2017 2,497 4 View

What is the best and easy and fast way to knockdown a gene in adult rat?

Dear RG-friends, I would like to knockdown a gene in adult rat and focusing first at a global level and then target a specific tissue/organ. Would you suggest me an alternative way to the...

09 September 2017 314 0 View

Cerament and cell therapy ?

Dear RG-friends, Would it be possible to use cerament for local cell therapy? I would be more than happy to know your opinion about it. Many thanks in advance. Best Regards, Mario 😉

08 August 2017 8,920 0 View

Can Dot Blot be of help when Western Blot does not work for certain Abs?

It was not possible to see any expected band for the target, with a very low molecular weight (8-12 kD), by WB. The membrane was very clean after incubation with Ab-I-AbII. Probably we lost...

03 March 2016 7,324 4 View

Freezing and culturing Human T-cells?

Please, Could you tell me what is the best condition (cell density/media..)to freeze and to culture human T-cells? How long can T-cells be in culture? Thank you in advance Mario

02 February 2016 5,720 3 View

What does the ratio phospholamban/SERCA (at message level) mean?

Several studies show the expression of SERCA and phospholamban at message levels. I have seen also that they have used the ratio of those genes. Could you help me to find the reason for it, please?

01 January 2016 8,210 2 View