Should 5% Oil Red O in propylene glycol stock be stored at room temperature?

Dear RGs, I have prepared 0.5 % Oil Red O (SIGMA) in propylene glycol. Should I keep the stock at 4 degrees or at room temperature? Thanks a lot in advance. Best Regards, Matio

01 February 2019 6,645 2 View

Does anybody know how to prepare sugar-stock solution for cell culture experiment?

Hi. I would like to prepare stock solution for glucose or galactose or lactose for cell culture experiment. I will be very happy if you can give me tips, such as: in what (PBS, medium) to dissolve...

06 July 2018 5,314 1 View

Has anyone experienced with GLUCOSE tolerance test in neonatal mice pups (P0-P1)?

I would like to make the test in mouse pups (P0/P1). I thought to use 2 ways: via gavage and IPGTT. 1) Could you suggest me the size of the tube and of the syringe that should be used for the...

05 June 2018 7,689 0 View

Has anyone experienced with LACTOSE tolerance test with mice?

Dear RG-friends, I would like to do a lactose tolerance test in mouse newborns by measurement of the blood glucose level with a normal glucometer. I am wondering if someone did it and if kindly...

05 June 2018 7,136 2 View

What is the best way to isolate total RNA from plasma?

Dear RG-friends, I would like to isolate total RNA from plasma. Many thanks in advance for your timing and consideration. Best Regards, Mario

03 April 2018 1,662 2 View

Does anyone have experience with smooth muscle cell transfection?

Dear RGs, I would like to run transfection experiments by using rat primary SMCs and I am wondering if someone can give advices about it. Thank you so much in advance. Best Regards, Mario

10 November 2017 2,453 4 View

What is the best and easy and fast way to knockdown a gene in adult rat?

08 September 2017 261 0 View

Can Dot Blot be of help when Western Blot does not work for certain Abs?

It was not possible to see any expected band for the target, with a very low molecular weight (8-12 kD), by WB. The membrane was very clean after incubation with Ab-I-AbII. Probably we lost...

02 March 2016 7,273 4 View

Freezing and culturing Human T-cells?

Please, Could you tell me what is the best condition (cell density/media..)to freeze and to culture human T-cells? How long can T-cells be in culture? Thank you in advance Mario

01 February 2016 5,634 3 View

What does the ratio phospholamban/SERCA (at message level) mean?

31 December 2015 8,131 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Your thoughts on the spherical densiometer to estimate relative species canopy cover?

Hi biologists and biogeomorphologists, I want to characterize vegetation plots on a transect that follows a successionnal gradient from a river bed to a mature forest. Ecologically, I am...

26 February 2021 5,720 2 View

What kind of institutional environment do entrepreneurs need to achieve happiness?

Entrepreneurs are stressful, and happiness is a scarce resource. Happiness is crucial to business development.In addition to the work characteristics, individual characteristics have an effect on...

25 February 2021 876 2 View

Any interests in participating to an experiment in space?

Hello, The UN Office of Outer Space Affairs has a program called access to space for all that enables institutions to have hands on expertise related to access to space. There are three elements...

22 February 2021 7,980 3 View

How much costs direct reduction of nitrogen on farmland in terms of lower crop yield?

Dear research fellows, I wonder if someone knows how much direct reduction of nitrogen on farmland costs in terms of lower crop yield expressed as EUR/kg nitrogen (or similar)? I understand that...

15 February 2021 1,323 3 View

It is easier to knockdown a gene than overexpress a gene when generate a stable cell line?

I am just so confused that some people tell me knockdown is easier than overexpression when generating a stable cell line. I think it depends on the gene. Cells won't be happy whether we try to...

15 February 2021 1,370 2 View

For acid treated montmorillonite clays what will be the result of surface area?

The organo montmorillonite nanoclays modified with octadecylamine were used for the experiment. After acid treatment, the surface area will be increase. However, in my experiment, the surface area...

10 February 2021 1,581 3 View

Which is the appropriate statistic test to examine the relation between students' affective state and academic performance?

Which test should I use to compare students' mood effects on academic performance? I want to compare mood measured on a likert scale (from point 1= very sad to point= 5 very happy) to three...

04 February 2021 1,907 5 View

Hello friends, happy evening !! I am just wondering if you would know where I can find the (ALTS) altruism scale by S.N.Rai and S Singh to download ?

ALTS is an altruism scale developed by S.N Rai and S.Singh.

02 February 2021 7,243 2 View

How to analyse multiple Likert scales?

Hello everyone! I've encountered a problem when trying to figure out the methodology of my dissertation. The study has multiple variables and will be, in general, rather complicated, but my only...

02 February 2021 2,086 3 View