Dear RG-friends,
Would it be possible to use cerament for local cell therapy?
I would be more than happy to know your opinion about it. Many thanks in advance. Best Regards, Mario 😉
Dear RGs, I have prepared 0.5 % Oil Red O (SIGMA) in propylene glycol. Should I keep the stock at 4 degrees or at room temperature? Thanks a lot in advance. Best Regards, Matio
01 February 2019 6,744 2 View
Hi. I would like to prepare stock solution for glucose or galactose or lactose for cell culture experiment. I will be very happy if you can give me tips, such as: in what (PBS, medium) to dissolve...
06 July 2018 5,400 1 View
Dear RG-friends, I would like to do a lactose tolerance test in mouse newborns by measurement of the blood glucose level with a normal glucometer. I am wondering if someone did it and if kindly...
05 June 2018 7,229 2 View
I would like to make the test in mouse pups (P0/P1). I thought to use 2 ways: via gavage and IPGTT. 1) Could you suggest me the size of the tube and of the syringe that should be used for the...
05 June 2018 7,770 0 View
Dear RG-friends, I would like to isolate total RNA from plasma. Many thanks in advance for your timing and consideration. Best Regards, Mario
03 April 2018 1,753 2 View
Dear RGs, I would like to run transfection experiments by using rat primary SMCs and I am wondering if someone can give advices about it. Thank you so much in advance. Best Regards, Mario
10 November 2017 2,535 4 View
Dear RG-friends, I would like to knockdown a gene in adult rat and focusing first at a global level and then target a specific tissue/organ. Would you suggest me an alternative way to the...
08 September 2017 356 0 View
It was not possible to see any expected band for the target, with a very low molecular weight (8-12 kD), by WB. The membrane was very clean after incubation with Ab-I-AbII. Probably we lost...
02 March 2016 7,359 4 View
Please, Could you tell me what is the best condition (cell density/media..)to freeze and to culture human T-cells? How long can T-cells be in culture? Thank you in advance Mario
01 February 2016 5,771 3 View
Several studies show the expression of SERCA and phospholamban at message levels. I have seen also that they have used the ratio of those genes. Could you help me to find the reason for it, please?
31 December 2015 8,266 2 View
Your partner’s not there to service you, it’s not their job to keep you sexually satisfied. You’re together because you love each other and want to make each other happy. Constantly hassling them...
08 August 2024 1,491 0 View
My case has been under treatment now for 3 years with little response. Now considering euthanasia. Last ditch is Cisplatin for dilution in iv infusion (100ml/ml in saline and mannitol). What can I...
05 August 2024 1,835 0 View
I want to know the cause of the elusive nature of happiness.
05 July 2024 4,668 0 View
I am working on a literature review of studies that systematically compare at least one case of collaborative governance to one or more cases of conventional governance (or that compare multiple...
25 June 2024 5,151 2 View
Dear Professor(s), I am looking for Post-Doc position or visiting researcher position on funded project based. If any professor has open position for international students, I will be happy to...
23 June 2024 9,619 1 View
Hi All, I'm currently completing my MRes research project on interoception. My research question is: Can interoceptive ability (i.e., accuracy, sensibility and metacognition) predict stress...
17 June 2024 7,399 0 View
This barrel or vase-shaped stony structure was found in the Cretaceous shales. Its outer surface was surrounded by loose and thick limonitic and clayey sheets before I removed them. After...
04 June 2024 5,914 5 View
I request your kind attention. I hope you all a happy day and successful days ahead. I am a researcher, studying the adoption factor of HR analytics in Indian industries. For this purpose, I...
16 May 2024 7,754 0 View
we find ourselves in need of a validated tool to measure happiness levels among our target population. Upon learning about the availability of the Hindi version of the Oxford Happiness...
15 May 2024 7,280 0 View
Dear All, I have some problems to get the stabilised fEPSP slope (baseline) for LTD. I had been measuring the maximum peak value at 200uA and baselining it at 30-40% of that value. However, I...
12 May 2024 9,612 0 View