Because some researches do autolysis of cells first, then treatment with the base, and others do the treatment with the base directly, which is better and why?
think you'll get a lot of cellular protein with whole cell extraction.
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Hello i need heavy oil PVT data having viscoisty of 2000-3000 cP. If someone have it or research papers about it, kindly share it with me Thanks
25 July 2024 6,940 1 View
I have an issue with SEM imaging of liposomes. When measured by DLS, they appear to be around 120 nm, but when using SEM with simple air drying, they appear much larger, around one micrometer.
25 July 2024 3,395 3 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 5 mg/mL?
18 July 2024 9,831 0 View
What is the protocol for storing phospholipids in chloroform solution? Should I divide the contents of a bottle into aliquots to prevent melting each time I take it out for an experiment from...
27 June 2024 2,138 2 View
in this link refractiveindex.info i didnt find i work on a simulation comsol of mesoporous perovskite i want to know the properties optoelectric and n,k coff
09 June 2024 2,071 4 View
Can rats that were injected with collagenase NPs, show higher collagen deposition than their control untreated counterparts in histopathology examination!!!
11 May 2024 2,931 0 View
I had Titanium Dioxide powder & I need a specific answer for both points:- What is the most suitable solnavents used to convert TiO2 powder to TiO2 solution What is the most suitable methods...
30 April 2024 5,931 1 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...
08 July 2024 9,201 2 View
The plasmid that was transformed originally is correct (sequenced). I am expressing different gene targets from the yeast genome to optimize my protein. When I sequence the incorrect size, it...
01 July 2024 2,356 6 View
Image attached is at 20X. Cultured media for checking presence of bacteria and yeast and both tests were negative.
27 June 2024 7,473 4 View
I am planning to measure the the activity of beta galactosidase enzyme using a standard kit to detect glucose production. The kit reagent is a mixture including horseradish peroxidase and...
12 June 2024 7,331 1 View
Which is the best method for the characterization of beta cyclodextrin complex formation with guest molecule in solution form and in solid form ?
11 June 2024 6,719 0 View
We have to report the concentration in pg/ml, the ELISA kit provider did not reveal the specific activity of the enzyme. In that case how to convert the units?
05 June 2024 2,173 5 View
Hello, community, Could you please clarify whether current legislation permits the reuse of algae biomass after it has been used to treat non-hazardous decontaminated laboratory organic waste?...
05 June 2024 5,040 1 View