First, I think aliquoting is alway a good idea to keep your material safe..

Chloroform remains liquid in the freezer anyway.

The fatty acid moieties from your phospholipids (PL) can be unsaturated, i.e. contain double bonds, which can react under the influence of UV, with Oxygen, water etc. But that depends on the PLs. In pure chloroform, they are fine.

Nonetheless, I keep PLs in chloroform in the -20 freezer and in brown glass bottles.

Thank you for the information. In my phospholipid formulation, I use DPPC, DSPE-PEG, and cholesterol, all of which are fully saturated.

Natascha Lewe