My edible films start shrinking and become softer and sticky immediately when they comes in touch with media.
Have you tried using them in a similar fashion to an antibiotic disk? Just cut a small piece and put on a plate seeded with bacteria and see if you get a zone of inhibition after the bacterial lawn forms. It shouldn't matter if the film dissolves.
Thank you Dr. Michael J. Benedik, yes I cut the films in disc shapes and did the experiment in a similar fashion as I used to do with antimicrobial discs. But when I put the films on media the films started shrinking and dissolving. Just I am worried about measuring the zone of inhibition, is it fine if the discs become deformed in shape after touching the media?
If it becomes deformed then there might be some differential amounts of contact and hence diffusion, that would be one concern. But I would just do a control experiment, take 4 or 5 identical disks and put them on plates and see if the zones are the same size or not. That will tell you if it is a reproducible and reliable measure. But you need to be sure the discs are the same size.
Thank you Dr. Michael J. Benedik,
I will try as you said. I hope it will give me results. I will contact you again after I get the results.
Thank you so much.
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