Using 10 ml of 1% methanolic hcl, 1 g fresh sample and OD at 530 and 657nm
To calculate the anthocyanin concentration in the fresh sample using the provided information, we need to follow these steps:
1. Determine the optical densities (OD) of the sample at the specified wavelengths (530 nm and 657 nm).
2. Calculate the average optical density (OD_avg) using the formula:
ODavg = OD530 + OD657 / 2
3. Calculate the change in optical density (ΔOD) using the formula:
ΔOD = ODavg - ODblank
4. Determine the anthocyanin concentration using the formula:
C = ΔOD \ ε x d
Given:
- Volume of methanolic HCl solution used (V) = 10 mL
- Concentration of methanolic HCl solution = 1%
- Weight of fresh sample (m) = 1 g
- Optical densities at 530 nm and 657 nm (OD_530 and OD_657)
- Molar absorptivity coefficient of anthocyanin (\( \varepsilon \)) = to be provided
- Path length of the cuvette (d) = to be provided
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