We are hoping to test a new drug for triple-negative breast cancer in a suitable animal model. What triple-negative cell line should we use?
Thank you
In our experiences with commercial MDA-MB-231 xenografting (http://altogenlabs.com/xenograft-models/breast-cancer-xenograft/mda-mb-231-xenograft-model/) the xenografts do have good growth patterns, but can be tricky to deal with. It does match your desired characteristics, and would be an excellent choice if you already know what kind of xenograft study you would like to run.
Hi,
I'd give MDA-MB-231 a try. They are triple-negative (claudin-low) and have been shown to be tumourigenic.
Kind regards
MDA-MB 436 ( BRCA negative TNBC ) implant in SCID mice
It would help you
MDA-MB-231 is certainly one of the most widely used cell lines, and has the advantage of spontaneously metastasizing from the mammary fat pad of nod/scid mice in orthotopic models; see, for example:
https://www.researchgate.net/profile/Evette_Radisky/publication/262191605_Tumor_cell-produced_matrix_metalloproteinase_9_MMP-9_drives_malignant_progression_and_metastasis_of_basal-like_triple_negative_breast_cancer/links/00b495374b9d1c4e88000000.pdf
These publications offer further information about preclinical models that recapitulate aspects of different TNBC subtypes:
https://www.researchgate.net/profile/Sophia_Ran/publication/232700661_Novel_Model_for_Basaloid_Triple-negative_Breast_Cancer_Behavior_In_Vivo_and_Response_to_Therapy_1_2/links/0fcfd50d32614df6fa000000.pdf
https://www.researchgate.net/profile/Bapsi_Chakravarthy/publication/51184457_Lehmann_BD_Bauer_JA_Chen_X_Sanders_ME_Chakravarthy_AB_Shyr_Y_Pietenpol_JAIdentification_of_human_triple-negative_breast_cancer_subtypes_and_preclinical_models_for_selection_of_targeted_therapies_J_Cli/links/004635225f37d3a16a000000.pdf
Article Tumor cell-produced matrix metalloproteinase 9 (MMP-9) drive...
Article Novel Model for Basaloid Triple-negative Breast Cancer: Beha...
Article Lehmann BD, Bauer JA, Chen X, Sanders ME, Chakravarthy AB, S...
Hi Michael,
MDA-MB-231 is easily available, fast growing, high invasive and familiar cell line to work with. I recommend the same.
Best,
S
Hi Michael,
MDA-MB-231 definitely, not only triple-negative, fast growing and invasive, but p53 mutant.
You'll probably need another (control) triple-negative, but p53 wild type cell line. I know that MCF-7 is p53 wild type, but express estrogen receptor. However, try to find if any of ATCC triple negative cell lines (https://www.lgcstandards-atcc.org/Products/All/TCP-1003?geo_country=rs) is p53 wild type.
Kind regards,
In our experiences with commercial MDA-MB-231 xenografting (http://altogenlabs.com/xenograft-models/breast-cancer-xenograft/mda-mb-231-xenograft-model/) the xenografts do have good growth patterns, but can be tricky to deal with. It does match your desired characteristics, and would be an excellent choice if you already know what kind of xenograft study you would like to run.
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