I am looking for polymers/biopolymers with high hydrophobicity and high gas permeability. Can any one recommend any types of polymers/biopolymers?

I am looking for polymers/biopolymers with high hydrophobicity and high gas permeability. Can any one recommend any types of polymers/biopolymers?

25 November 2020 5,887 3 View

How to use Incremental/Online learning in face recognition?

I am trying to implement incremental/online learning for a facial recognition application. I've trained a model on a dataset and it works perfectly fine, however, I need to capture new...

23 September 2020 3,819 5 View

How to Fill Up volume in CST MWS?

How to Fill Up volume in CST MWS Hello everybody, Does Anyone know how to fill up a generic volume with a certain material using CST Microwave Studio Software? How to connect a curved plate to...

07 February 2020 4,427 1 View

How to use a CNN model saved as .mat file in python?

Hello everyone, I am going to use a FER2013 fine-tuned pretrained VGG-Face model to extract some features from facial images. Actually, I have the weights and layers of this model saved as a mat...

21 January 2019 4,387 2 View

Which CD20 expressing cell line is better?

Hi there , we are working on antibodies against CD20 antigen ( tositumomab, ofatumumab, Obinutuzumab ) and need a cell line which overexpressing CD20 . as far as I am concerned, Daudi , Ramos...

07 November 2018 1,015 3 View

Can I use SLA1 instead of XHO1?

Hi there. I want to know is it possible to use SLA1 Enz instead of XHO1? My gene of interest is CD20 And I'm working on PSpax, PMD2G plasmid and PCDH. Any suggestion or comment would be...

31 August 2018 7,838 3 View

PCR troubleshooting.what should I do to solve the problem ?

29 August 2018 1,651 6 View

Any suggestion or comment in regard of PCR troubleshooting ?

20 August 2018 150 10 View

Hi.How much blood is needed to gain enough lymphocytes to do RNA extraction?

How many PBMC can be isolated from 10 ml of blood from a healthy donor?

19 July 2018 7,153 3 View

Why does my Blue native gel doesn't run completely?

I have been running blue native gels for my chloroplast samples, the problem is that the gel won't run completely. I have seen gel pictures in different papers that the whole sample runs really...

15 January 2018 4,291 2 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View