Niloufar Mohammadkhani

7 Questions 41 Answers 0 Followers

Questions related from Niloufar Mohammadkhani

Which CD20 expressing cell line is better?

Hi there , we are working on antibodies against CD20 antigen ( tositumomab, ofatumumab, Obinutuzumab ) and need a cell line which overexpressing CD20 . as far as I am concerned, Daudi , Ramos...

08 November 2018 1,159 3 View

Can I use SLA1 instead of XHO1?

Hi there. I want to know is it possible to use SLA1 Enz instead of XHO1? My gene of interest is CD20 And I'm working on PSpax, PMD2G plasmid and PCDH. Any suggestion or comment would be...

01 September 2018 8,000 3 View

What is the best protocol for competent cell preparation ?

Hello there. I'm working on E-coli(top-10). I would like to know What is the best protocol for competent cell preparation? Any suggestion in this regard would be appreciated. Thanks Niloo

01 September 2018 6,937 4 View

PCR troubleshooting.what should I do to solve the problem ?

Hi, I recently ran a PCR Reaction using Taq 2x Master Mix RED - Ampliqon The primers I used were both 10µM. I added 2µl same template human cDNA and diluted to 10µl total with water. The cycle...

30 August 2018 1,802 6 View

Any suggestion or comment in regard of PCR troubleshooting ?

Hi, Here is my PCR Reaction The primers I used were both 10µM. I added 2µl template cDNA and diluted to 10µl total with water. The cycle used was as follows: 95c

21 August 2018 303 10 View

Hello. Previous recommendations on SFA consumption, including animal fat, has changed?

Does the new research confirm the previous findings about the high percentage of saturated fatty acids in the diet and its hazards and complications? I am interested in finding out more about in...

22 July 2018 2,961 6 View

Hi.How much blood is needed to gain enough lymphocytes to do RNA extraction?

How many PBMC can be isolated from 10 ml of blood from a healthy donor?

20 July 2018 7,316 3 View