Hi dear researchers
I need to calculate the insert mass for the ligation reaction, the vector length is 7800bp and the insert length is 83bp, would you please guide me what is the correct and suitable insert/vector ratio? my insert is very small in comparison to vector
I used 10;1 , but I did not obtain my results.
I appreciate you for taking the time.
When you talk about ratio, are you referring to mass (ng's) or to molarity (nm)? What is relevant is the molarity or actually number of molecules. So if you added 10ng each of vector and insert, you would have a ratio of molecules that is about 1:100 (vector:insert because they are nearly 100-fold difference in size). Ideally you would want a molar ratio around 1:3 to 1:5 depending upon the sites etc.
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