Can a mutation created by CRISPR in an exon be inherited only at genomic level & the gene is transcribed normally (w/o mutation) in f3 generation?

I have generated a 20 bp deletion in an exon of gene X in zebrafish. The mutation is inherited. Screened for x-/- by PCR/ sequencing and crossed the positive fish (F2 generation). Their progeny...

18 June 2019 8,821 10 View