Should I use Dunnett's multiple comparison test?

The attached graph shows the expression of a protein (Y-axis) at each cell division (X-axis) in three different treatment groups. I would like to compare the red and orange groups (test) to the...

10 November 2017 6,580 3 View

Should the B16 melanoma cancer line be transplanted to male or female mice?

01 February 2015 5,043 20 View

How does a 670 LP (long pass) filter work in a FACS machine? And is it a problem?

The FACS machine at our core facility has a 670 LP (long pass) filter to detect PerCP and PerCP-Cy5.5. Yet, unlike the band pass (BP) filters these seem to accept a large chunk of the emission...

01 February 2015 1,754 6 View

What are the typical lung function assays in mice?

What type of assays can one do to measure lung function in mice?Can you measure oxygenation of blood? Breathing volume? Inflation of the lungs?

31 December 2014 7,702 9 View

What cell types are affected when Cre is expressed from the distal Lck promoter?

31 December 2014 1,616 3 View

Is it possible to measure telomere length using flow cytometry?

Is there a method to stain cells with a fluorescent antibody or molecule that directly or indirectly measures telomere length?

04 May 2014 2,240 7 View

Do EL4 thymoma cells express PDL-1 (CD274)?

Has anyone measured surface expression of PDL-1 on EL4 cells? And while I am it: do they produce TGF-beta? Just wondering what kind of immunosuppressive "arsenal" EL4 cells have to fight off...

31 December 2013 6,802 10 View

Can the lox-cre system be used with retroviral vectors?

The idea would be to use retroviral vectors bearing LoxP sites to infect cells expressing inducible Cre recombinase (under control of Dox or Tamoxifen). This would be used to delete transgene...

05 June 2013 5,178 9 View

What are the serum kinetics of doxycycline in mice when administered in drinking water?

I am currently using an inducible system for gene expression in mice and would like to know how long and at what serum concentrations doxycycline persists in the organism.

04 May 2013 457 6 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

What are the options for global scale hydrology DEM?

Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....

18 February 2021 5,109 5 View

Can I use a categorical covariate in a Generalized Linear Model in SPSS v.26?

Hello everyone, hope you are well. I am planning to use a Generalized Linear Model in SPSS. I would like to add gender to the model as a covariate, however from what I have read, only continuous...

14 February 2021 8,937 4 View