Should I use Dunnett's multiple comparison test?

The attached graph shows the expression of a protein (Y-axis) at each cell division (X-axis) in three different treatment groups. I would like to compare the red and orange groups (test) to the...

10 November 2017 6,580 3 View

What is the best myeloablative conditioning regimen using chemotherapy for mice?

02 March 2016 943 2 View

Should the B16 melanoma cancer line be transplanted to male or female mice?

01 February 2015 5,043 20 View

How does a 670 LP (long pass) filter work in a FACS machine? And is it a problem?

The FACS machine at our core facility has a 670 LP (long pass) filter to detect PerCP and PerCP-Cy5.5. Yet, unlike the band pass (BP) filters these seem to accept a large chunk of the emission...

01 February 2015 1,754 6 View

What are the typical lung function assays in mice?

What type of assays can one do to measure lung function in mice?Can you measure oxygenation of blood? Breathing volume? Inflation of the lungs?

31 December 2014 7,702 9 View

Is it possible to measure telomere length using flow cytometry?

Is there a method to stain cells with a fluorescent antibody or molecule that directly or indirectly measures telomere length?

04 May 2014 2,240 7 View

Do EL4 thymoma cells express PDL-1 (CD274)?

Has anyone measured surface expression of PDL-1 on EL4 cells? And while I am it: do they produce TGF-beta? Just wondering what kind of immunosuppressive "arsenal" EL4 cells have to fight off...

31 December 2013 6,802 10 View

Can the lox-cre system be used with retroviral vectors?

The idea would be to use retroviral vectors bearing LoxP sites to infect cells expressing inducible Cre recombinase (under control of Dox or Tamoxifen). This would be used to delete transgene...

05 June 2013 5,178 9 View

What are the serum kinetics of doxycycline in mice when administered in drinking water?

I am currently using an inducible system for gene expression in mice and would like to know how long and at what serum concentrations doxycycline persists in the organism.

04 May 2013 457 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

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03 March 2021 5,360 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

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02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View