Pedro Veliça

10 Questions 27 Answers 0 Followers

Questions related from Pedro Veliça

Should I use Dunnett's multiple comparison test?

The attached graph shows the expression of a protein (Y-axis) at each cell division (X-axis) in three different treatment groups. I would like to compare the red and orange groups (test) to the...

11 November 2017 6,627 3 View

What is the best myeloablative conditioning regimen using chemotherapy for mice?

I have routinely conditioned C57BL6 mice with 5.5 Gy of total body irradiation (TBI) to achieve non-lethal temporary myeloablation.What would be an equivalent chemotherapy regimen?

03 March 2016 990 2 View

Should the B16 melanoma cancer line be transplanted to male or female mice?

Does the gender of the recipient animal matter for the engraftment of the B16 tumour line? What is the gender of the cell line? I have seen publications were males were used and others were female...

02 February 2015 5,100 20 View

How does a 670 LP (long pass) filter work in a FACS machine? And is it a problem?

The FACS machine at our core facility has a 670 LP (long pass) filter to detect PerCP and PerCP-Cy5.5. Yet, unlike the band pass (BP) filters these seem to accept a large chunk of the emission...

02 February 2015 1,863 6 View

What cell types are affected when Cre is expressed from the distal Lck promoter?

The distal Lck promoter is active in T cells in the late stages of thymic maturation but does that include CD8, CD4 and gamma-delta T cells? Or are gd T cells left out?

01 January 2015 1,659 3 View

What are the typical lung function assays in mice?

What type of assays can one do to measure lung function in mice?Can you measure oxygenation of blood? Breathing volume? Inflation of the lungs?

01 January 2015 7,742 9 View

Is it possible to measure telomere length using flow cytometry?

Is there a method to stain cells with a fluorescent antibody or molecule that directly or indirectly measures telomere length?

05 May 2014 2,284 7 View

Do EL4 thymoma cells express PDL-1 (CD274)?

Has anyone measured surface expression of PDL-1 on EL4 cells? And while I am it: do they produce TGF-beta? Just wondering what kind of immunosuppressive "arsenal" EL4 cells have to fight off...

01 January 2014 6,848 10 View

Can the lox-cre system be used with retroviral vectors?

The idea would be to use retroviral vectors bearing LoxP sites to infect cells expressing inducible Cre recombinase (under control of Dox or Tamoxifen). This would be used to delete transgene...

06 June 2013 5,224 9 View

What are the serum kinetics of doxycycline in mice when administered in drinking water?

I am currently using an inducible system for gene expression in mice and would like to know how long and at what serum concentrations doxycycline persists in the organism.

05 May 2013 506 6 View