What type of assays can one do to measure lung function in mice?
Can you measure oxygenation of blood? Breathing volume? Inflation of the lungs?
whole body plethysmometer is generally use for various Lung function test, whereas the PowerLab | ADInstruments, for rat and mice are use for various invasive parameters in lung and cardiac model.
This link will help you to find your answer, and oxygenation of blood can be measured by simple method and by the simple instrument name as Pulse oximetry.
https://www.google.co.in/url?sa=t&rct=j&q=&esrc=s&source=web&cd=4&cad=rja&uact=8&ved=0CDIQFjAD&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC2039738%2F&ei=A6e3VN2EGs-yuASa_oD4Bg&usg=AFQjCNHvvRi3-i-MoLqB4_P6GEsoOn0TmQ&bvm=bv.83640239,d.c2E
You can measure a host of different measurements with a machine called a FlexiVent (made by Scireq), including pressure volume curves, resistance and compliance (all different measures of inflation and lung volume). Pulse oximetry and treadmill tests will give you a better understanding of the actual function as opposed to only the lung mechanics.
Hi, KI has a facility for lung mechanics (Flexivent) in small animals. Talk to Sven-Erik Dahléns research group at IMM /Sofia
Pedro I think you have some good advice. Typically, pressure-volume curves (lung mechanics) are measured and the measurement depends on what you are looking for in the mice. I am unaware if anyone has developed an oscillometer for small animals but it is certainly used in people and larger animals. Will measure airway resistance, reactance and inertance as well as a few other parameters. As for oxygenation a small sample of blood and run an ABG may yield what you are looking. Sampling site may be problematic. Oximetry can be used or TcPO2. I also think that perhaps measuring exercise ability may yield some important information but this again depends on the question and what the mice have experienced.
Hi Pedro
A PubMed search for Peter Sly and Zoltan Hantos will bring up some useful mouse lung function studies using the Forced Oscillation Technique. Wayne Mitzner is also very good in this area, see http://www.ncbi.nlm.nih.gov/pubmed/17868442. Be careful of Penh (from whole body plethysmography), it is often used but not well accepted in the lung function field (Sly and Hantos have publications about that as well). You can measure lung function in mice using FOT in mice from when they are very small, but it can be tricky (see Bozanich et al. http://www.ncbi.nlm.nih.gov/pubmed/17495115)
Good luck
Hi Pedro. Depending on the model you want to apply there are several methods:
Whole Body Pletshymosgraphy, Forced Manouvers, Resistance and Compliance and so forth.
Do you want to perform longitudinal studies on your mice (several repetition)? Or can the study be terminal?
Of course with Resistance and Compliance and Forced Manouver you have terminal studies. What we generally do is first Whole body plethysmography for screening and then go to the most sophisticated Resistance and Compliance which are terminal studies (e.g. for Asthma Models).
A relative easy to use system in the sense that can self calibrate and you can have a skilled technical sitting there while you get the data right away analysis is the Buxco System.
Good Luck.
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