What is the best method to stain Zebrafish embryos?

More Archisman Mahapatra's questions See All

Is there any drugs or natural ingrdients with similar mechanism like that of low dose Amisulpride?

Is there any approved drugs or natural ingredients with similar mechanism like that of low dose Amisulpride. Is there any specific dose or borderline dose reported at which amisulpride is both...

02 June 2024 5,586 0 View

How to publish a paper in the journal "CELL" ?

Is Cell the unit of living organism / life? Absolutely incorrect. Explanation: From fossils cell we have estimated , before how many years it was there, e.g 40millions years etc.. Was that cell...

28 May 2024 5,931 1 View

How can we use cyclic voltammetry for characterization of Microbial Fuell Cell?

I have been using the traditional method for characterizing microbial fuel cell where at the end we will obtain power density curve (Power density vs Current) and polarization curve ( Voltage vs...

14 February 2024 957 0 View

Why difeerentiated SH-SY5Y cells (10 uM RA for 3 days) showing MAP2a and MAP2b bands in western blot ?

I did western blot to check MAP2 expression in differentiated SHSY5Y cells. I followed different differentiation method (10 uM RA for 3 days, 10 uM RA for 7 days, 5 uM RA for 3 days and 5 uM RA...

11 October 2023 9,726 0 View

How can I create a 3% CO2 atmosphere within a CO2-free incubator?

My laboratory lacks a CO2 incubator, and I'm working with cyanobacteria. Are there any suggestions for introducing CO2? Furthermore, how can I ensure the precise 3% CO2 concentration?

18 August 2023 6,059 3 View

Whatever you have learned since childhood to till yet, have you learned it by yourself or someone has taught you?

Whatever you have learned since childhood to till yet, have you learned it by yourself or someone has taught you? If Yes, who taught you? If No, how you learned?

03 March 2023 5,481 4 View

96 well plate for estimation of NADH concentration at 340nm?

I am trying to perform a biochemical assay which requires me to take readings for reduction in NADH levels at 340 nm. Could anyone kindly suggest what plates should I be using. I currently have...

12 October 2022 798 2 View

Canonical SMILE of a polymer structure?

Can anyone help me by providing canonical SMILE of polystyrene polymer of different chain length?

27 August 2022 398 0 View

During cross coupling reaction which part I should choose as aryl halide and which part as boronic ester part in Suzuki cross coupling reaction?

Actually I am not able to understand that during the synthesis of a molecule if I do disconnection and try to make the molecule by cross coupling reaction then which part I should consider as...

15 August 2022 8,799 1 View

How to load a single file of 3827 molecules in pdb format into PyRx?

I have a single file of 3827 molecules in pdb format. When I am loading the file into pyrx, it can't load the molecules. The remaining time shown 699 hours and keep on increasing. My laptop has 8...

07 July 2022 6,792 1 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

Best method for extracting RNA from morulae?

Best method and number of embryos for extracting RNA from pig morulae?

21 July 2024 4,398 1 View

Can anyone recommend a brand of cas9 protein that can be used for in-vivo editing in model animals?

zebrafish and mouse

19 July 2024 9,158 1 View

How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...

14 July 2024 6,240 0 View

How to Freeze Embryos for Spatial Transcriptomics?

Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...

10 July 2024 5,985 0 View

Why is my zebrafish sample falling out of the agarose embedding?

Hi all, I am looking for advice on what could be going wrong with my zebrafish caudal fin tissue fixation that would cause the sample to fall out of the embedding while cryosectioning. During cryo...

25 June 2024 4,602 0 View

How many grams or milligrams of Artemia should be given to 6 dpf larvae?

Feeding zebrafish larvae properly is a challenge if we need to grow them. According to standard guidelines, how many grams or milligrams of Artemia should be given to 6 dpf larvae?

13 June 2024 389 0 View

Hey #zebrafish friends, does anyone know of some fake, reusable embryos that can be reused in a laboratory classroom setting for practice?

I want to teach a class using zebrafish embryos. It's in the afternoon, so I want to get my hands on some fake, reusable embryos that students can use for practice. Does anyone know of any?

10 June 2024 3,956 1 View

Where can I get some Bipotent Mouse Embryonic Liver (BMEL) cells ?

Reference for BMEL cells : Inducible differentiation and morphogenesis of bipotential liver cell lines from wild-type mouse embryos Hélène Strick-Marchand, Mary C Weiss doi:...

29 May 2024 7,198 0 View

Differential gene expression?

Hello, I am working on the mouse lens, in which I have obtained lens fiber and epithelium at two-time points, E17 (embryonic day 17) and P2 (postnatal day 2) for two different mouse strains,...

29 May 2024 3,367 0 View