All the experimental results need to be presented with statistical analysis. In case of adsorption isotherm and kinetic study, what does I include?

What are the things should I include under statistical analysis.

19 November 2020 5,098 2 View

How to clone a promoter region of a gene within pBI101.2 vector for GUS assay?

I am cloning about 1.5kb promoter of a gene within pBI101.2 vector for GUS assay. During the primer designing, I am facing a problem. Should I include some portion(say about +20 to +30 from the...

10 June 2019 6,950 2 View

Need a protocol for SOD, Catalase and LPO enzyme assay in 96 well plate?

I need detailed protocol. Thanks in advance.

20 June 2018 6,887 3 View

What are the methods to follow if I want to study cross talk between molecular pathways?

If I want to study the cross talks between pathways involving more than 20-30 genes, what are the methods I should follow? qRT PCR and then validation by Western Blot or Immunohistochemistry is...

19 June 2018 7,134 2 View

No title

19 June 2018 6,121 3 View

How to plot in-plane vibration modes of square plate using MATLAB. The plot looks like a distrorted X-Y plane grid ?

I have the natural frequencies and the related displacement equations in x, y directions for in-plane vibration. Every point in the plate has a displacement in x (denoted by u(x,y) ) and y...

30 October 2017 304 11 View

How to check purity of mono-/bis-/metal complex-azo dyes using UV-Vis Spectroscopy?

How to check purity of mono-/bis-/metal complex-azo dyes using UV-Vis Spectroscopy?

22 October 2017 3,767 6 View

Given a bare-Lagrangian for a renormalizable, interacting system, is the renormaized version unique? If yes, then which ?

It is well-known that several renormalized versions of a given theory may emerge depending upon the chosen regularization method, choice of subtraction scale/scheme,...

09 March 2017 8,192 40 View

Perovskites are a class of compounda, than how can we identify that which material is suited for harvesting solar energy?

26 February 2017 4,620 3 View

Between ER and SQ grade reagents of Qualigens (Thermo fisher) which one is analytical grade [AR] reagent ?

23 November 2016 716 7 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Which polarizers are usually used to measure the birefringence of zebrafish muscles?

I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...

23 February 2021 1,934 1 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

Is there any free software for behaviour analysis of zebrafish ?

I need to do novel tank test and light/dark test for zebrafish. So I need a tracking software for tracking in these tests. Is there any free softwares available for tracking of zebrafish ?

16 February 2021 1,541 1 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

Has anyone used a premade hybridization buffer (from Roche for example) for zebrafish in situ hybridizations?

Our in situs (alkaline phosphatase based) have stopped working. Probes are fine, but or homemade hybridization buffer seems to be the issue. Using the same supplier of deionized formaminde as...

03 February 2021 8,540 2 View

Can any tell me or suggest any research article or review about the concentration of peptide dissolve in system water and given to Adult Zebrafish?

Hello, everyone! I have chemically synthesized antioxidant peptide isolated from the plant. I have to validate its bioactivities in Adult zebrafish. I have to administer this peptide to zebra...

01 February 2021 5,809 3 View

Has anybody a protocol to determine the AChE activity in larvae zebrafish brain?

Even following the instruction of Karnovsky and Roots (1964), I still do not know how long I should fix the larvae and in what fixative. Could anyone help me? Thank you!

14 January 2021 385 2 View

Does anyone know what happened to these fish embryo samples in 4% PFA in PBS?

I'm trying to whole mount in situ hybridization in fish embryos. I fixed fish embryos in 4% PFA in PBS for 2days. And I found some sharp brown spots in embryo. I've never found that spots...

12 January 2021 2,722 2 View

Anti-PCNA antibody [PC10] to detect proliferating cells in the brain of adult zebrafish (sections)?

Hello, I am trying to mark cells undergoing proliferation in the brain of adult zebrafish (Danio rerio). I did immunohistochemistry on sections using the Anti-PCNA antibody [PC10] (ab29) with...

14 December 2020 4,989 1 View