Hello everyone,

I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we are following:

We have attached an image showing our current results. When we cut the embryos on the cryostat, the tissue tends to break, especially in the brain and liver areas.

Does anyone have suggestions or tips on how to improve our cryosectioning technique to prevent tissue damage? Your insights would be greatly appreciated.

Thank you!