What is the best method to find out a piece of DNA has transferred using bacteriophage or plasmid?

More Mushtaq Talib shawi AL- Rubaye's questions See All

Is there any bioinformatics software that can analyze several gene sequences for several samples simultaneously?

I want to analyze several genes for a set of bacteria simultaneously. Is it possible to do an analyze for that set of genes in a real time and drawing their phylogenetic tree, I mean that putting...

06 July 2017 7,123 4 View

Can I send no band 1 for sequencing?

In this pic I want to send no: 1 for sequencing but i think its smear will be a big problem. Please advise me.

02 March 2015 4,553 16 View

What is the best technique for PCR products purification for samples with protein impurities?

My template DNA has high quantity of protein impurities I did PCR and get a good band after gel electrophoresis but I saw a smear in the gel I have checked the DNA purity with nanodrop from the...

31 December 2014 6,635 7 View

What is the best protocol for 16sr RNA gene PCR?

I have problem when I want to PCR 16sr RNA gene of my unknown strain. I have extracted its DNA and did PCR by using lyophilized master mix tube for PCR. The result was good but I had an extra band...

31 December 2014 1,301 11 View

What is the best technique for Long-term storage of bacterial culture?

Except lyophilization what is the best why for bacterial culture storage?

31 December 2014 9,156 13 View

How can we detect the flagella by microscopic techniques?

I want to see if isolated bacteria has flagella or not. But I want to do it by using a microscope and without staining. Is there any technique that can help me?

11 December 2014 8,565 3 View

Why some time after DNA extraction there is no band in electrophoresis?

I extracted DNA from bacteria by using extraction Kit, by when I run the DNA on agarose gel no DNA band detected, but when I did the PCR reaction I got good result. And when I run it on agarose...

11 December 2014 4,132 9 View

How can I extract and purify cloned DNA polymerase?

Is there any protocol to extract and purify cloned DNA polymerase from bacteria?

11 December 2014 7,060 4 View

What is a suitable thermal program for 16s rRNA gene PCR in halophilic bacteria?

What is a suitable thermal program for 16s rRNA gene PCR in halophilic bacteria? Usually halophilic bacteria have high % of G+C content and this affects the thermal program for PCR. I would like a...

11 December 2014 9,661 2 View

What is the best molecular test for describing novel subspecies of bacteria?

when someone want to describing a novel bacterial subspecies , he or she asked to do extra testes such as molecular, which testes are the best and necessary for this case?

10 November 2014 372 6 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View