Is there any bioinformatics software that can analyze several gene sequences for several samples simultaneously?

I want to analyze several genes for a set of bacteria simultaneously. Is it possible to do an analyze for that set of genes in a real time and drawing their phylogenetic tree, I mean that putting...

06 July 2017 7,042 4 View

Can I send no band 1 for sequencing?

02 March 2015 4,465 16 View

What is the best protocol for 16sr RNA gene PCR?

I have problem when I want to PCR 16sr RNA gene of my unknown strain. I have extracted its DNA and did PCR by using lyophilized master mix tube for PCR. The result was good but I had an extra band...

31 December 2014 1,220 11 View

What is the best technique for PCR products purification for samples with protein impurities?

My template DNA has high quantity of protein impurities I did PCR and get a good band after gel electrophoresis but I saw a smear in the gel I have checked the DNA purity with nanodrop from the...

31 December 2014 6,553 7 View

What is the best technique for Long-term storage of bacterial culture?

Except lyophilization what is the best why for bacterial culture storage?

31 December 2014 9,055 13 View

How can we detect the flagella by microscopic techniques?

11 December 2014 8,482 3 View

Why some time after DNA extraction there is no band in electrophoresis?

I extracted DNA from bacteria by using extraction Kit, by when I run the DNA on agarose gel no DNA band detected, but when I did the PCR reaction I got good result. And when I run it on agarose...

11 December 2014 4,004 9 View

How can I extract and purify cloned DNA polymerase?

Is there any protocol to extract and purify cloned DNA polymerase from bacteria?

11 December 2014 6,982 4 View

What is a suitable thermal program for 16s rRNA gene PCR in halophilic bacteria?

What is a suitable thermal program for 16s rRNA gene PCR in halophilic bacteria? Usually halophilic bacteria have high % of G+C content and this affects the thermal program for PCR. I would like a...

11 December 2014 9,565 2 View

What is the best molecular test for describing novel subspecies of bacteria?

10 November 2014 263 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View