15 Questions 27 Answers 0 Followers
Questions related from Mushtaq Talib shawi AL- Rubaye
I want to analyze several genes for a set of bacteria simultaneously. Is it possible to do an analyze for that set of genes in a real time and drawing their phylogenetic tree, I mean that putting...
07 July 2017 7,095 4 View
What is the best method to find out a piece of DNA has transferred using bacteriophage or plasmid?
02 February 2017 425 2 View
In this pic I want to send no: 1 for sequencing but i think its smear will be a big problem. Please advise me.
03 March 2015 4,525 16 View
I have problem when I want to PCR 16sr RNA gene of my unknown strain. I have extracted its DNA and did PCR by using lyophilized master mix tube for PCR. The result was good but I had an extra band...
01 January 2015 1,272 11 View
Except lyophilization what is the best why for bacterial culture storage?
01 January 2015 9,118 13 View
My template DNA has high quantity of protein impurities I did PCR and get a good band after gel electrophoresis but I saw a smear in the gel I have checked the DNA purity with nanodrop from the...
01 January 2015 6,605 7 View
I want to see if isolated bacteria has flagella or not. But I want to do it by using a microscope and without staining. Is there any technique that can help me?
12 December 2014 8,541 3 View
What is a suitable thermal program for 16s rRNA gene PCR in halophilic bacteria? Usually halophilic bacteria have high % of G+C content and this affects the thermal program for PCR. I would like a...
12 December 2014 9,627 2 View
Is there any protocol to extract and purify cloned DNA polymerase from bacteria?
12 December 2014 7,031 4 View
I extracted DNA from bacteria by using extraction Kit, by when I run the DNA on agarose gel no DNA band detected, but when I did the PCR reaction I got good result. And when I run it on agarose...
12 December 2014 4,101 9 View
Is there any journal except IJSEM that accept papers include description of novel bacterial species?
11 November 2014 4,410 1 View
in case of testing ligninase for an individual bacterial strain how we can test ligninase activity? i mean i need a formula for medium and its protocol. there are several protocols for screening...
11 November 2014 6,732 2 View
antagonism test protocol for bacteria. searching for antibiotics and bactericides produced by bacteria
11 November 2014 7,963 3 View
when someone want to describing a novel bacterial subspecies , he or she asked to do extra testes such as molecular, which testes are the best and necessary for this case?
11 November 2014 341 6 View
I need a list of best RAPD primers for bacteria.
11 November 2014 9,183 4 View
