Is there any bioinformatics software that can analyze several gene sequences for several samples simultaneously?

I want to analyze several genes for a set of bacteria simultaneously. Is it possible to do an analyze for that set of genes in a real time and drawing their phylogenetic tree, I mean that putting...

06 July 2017 7,042 4 View

What is the best method to find out a piece of DNA has transferred using bacteriophage or plasmid?

What is the best method to find out a piece of DNA has transferred using bacteriophage or plasmid?

01 February 2017 371 2 View

Can I send no band 1 for sequencing?

02 March 2015 4,465 16 View

What is the best protocol for 16sr RNA gene PCR?

I have problem when I want to PCR 16sr RNA gene of my unknown strain. I have extracted its DNA and did PCR by using lyophilized master mix tube for PCR. The result was good but I had an extra band...

31 December 2014 1,220 11 View

What is the best technique for PCR products purification for samples with protein impurities?

My template DNA has high quantity of protein impurities I did PCR and get a good band after gel electrophoresis but I saw a smear in the gel I have checked the DNA purity with nanodrop from the...

31 December 2014 6,553 7 View

What is the best technique for Long-term storage of bacterial culture?

Except lyophilization what is the best why for bacterial culture storage?

31 December 2014 9,055 13 View

How can we detect the flagella by microscopic techniques?

11 December 2014 8,482 3 View

Why some time after DNA extraction there is no band in electrophoresis?

I extracted DNA from bacteria by using extraction Kit, by when I run the DNA on agarose gel no DNA band detected, but when I did the PCR reaction I got good result. And when I run it on agarose...

11 December 2014 4,004 9 View

What is a suitable thermal program for 16s rRNA gene PCR in halophilic bacteria?

What is a suitable thermal program for 16s rRNA gene PCR in halophilic bacteria? Usually halophilic bacteria have high % of G+C content and this affects the thermal program for PCR. I would like a...

11 December 2014 9,565 2 View

What is the best molecular test for describing novel subspecies of bacteria?

10 November 2014 263 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View