Is there any bioinformatics software that can analyze several gene sequences for several samples simultaneously?

I want to analyze several genes for a set of bacteria simultaneously. Is it possible to do an analyze for that set of genes in a real time and drawing their phylogenetic tree, I mean that putting...

06 July 2017 7,042 4 View

What is the best method to find out a piece of DNA has transferred using bacteriophage or plasmid?

What is the best method to find out a piece of DNA has transferred using bacteriophage or plasmid?

01 February 2017 371 2 View

Can I send no band 1 for sequencing?

02 March 2015 4,465 16 View

What is the best protocol for 16sr RNA gene PCR?

I have problem when I want to PCR 16sr RNA gene of my unknown strain. I have extracted its DNA and did PCR by using lyophilized master mix tube for PCR. The result was good but I had an extra band...

31 December 2014 1,220 11 View

What is the best technique for PCR products purification for samples with protein impurities?

My template DNA has high quantity of protein impurities I did PCR and get a good band after gel electrophoresis but I saw a smear in the gel I have checked the DNA purity with nanodrop from the...

31 December 2014 6,553 7 View

What is the best technique for Long-term storage of bacterial culture?

Except lyophilization what is the best why for bacterial culture storage?

31 December 2014 9,055 13 View

How can we detect the flagella by microscopic techniques?

11 December 2014 8,482 3 View

Why some time after DNA extraction there is no band in electrophoresis?

I extracted DNA from bacteria by using extraction Kit, by when I run the DNA on agarose gel no DNA band detected, but when I did the PCR reaction I got good result. And when I run it on agarose...

11 December 2014 4,004 9 View

How can I extract and purify cloned DNA polymerase?

Is there any protocol to extract and purify cloned DNA polymerase from bacteria?

11 December 2014 6,982 4 View

What is the best molecular test for describing novel subspecies of bacteria?

10 November 2014 263 6 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View