I want to see if isolated bacteria has flagella or not. But I want to do it by using a microscope and without staining. Is there any technique that can help me?
Darkfield microscopy is a simple technique to enhance the contrast of very thin structures without the need for staining. We use this for the imaging of platelet filopodia. This could work for your question too. Regards. Max
Darkfield microscopy is a simple technique to enhance the contrast of very thin structures without the need for staining. We use this for the imaging of platelet filopodia. This could work for your question too. Regards. Max
Here is a link for some useful information gathered on Olympus site for Differential Interference Microscopy (DIC) http://www.olympusmicro.com/primer/techniques/dic/dichome.html See also comparison of DIC microscopy and phase contrast microscopy in this link - both used for visualising flagella. If you search PubMed for flagella visualisation there is quite a few papers and chapters which have a lot of useful practical information. Good luck with your project.
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