Kiran Sreeja Jayan

5 Questions 44 Answers 0 Followers

Questions related from Kiran Sreeja Jayan

His- Protein precipitation during buffer exchange?

Hi all, I am having issues with protein precipitation during buffer exchange. His-tag protein is eluted in 20mM HEPES, 200mM imidazole, 500mM NaCl at pH8.0. During elution, the tube with the...

06 June 2017 5,116 11 View

Protein precipitation during buffer exchange?

Hi all, I noticed small white precipitates formed during buffer exchange using Vivaspin. I am new to protein biology. I am not sure if the white stuff that I see are protein precipitates or...

08 August 2016 405 10 View

Can I use DMINDA for scanning whole genome sequence of a prokaryote to identify transcription factor binding sites?

Hi, I am not very good at computational biology. I am working on a transcriptional repressor protein which is believed to be associated with virulence. I did DNAse 1 footprinting to determine the...

07 July 2016 9,306 4 View

Genomic DNA extraction - What is the white pellet like thing after elution of extracted genomic DNA using Roche high PCR Template preparation kit?

I have extracted the bacterial genomic DNA using Roche High PCR Template preparation kit. After elution with elution buffer, I had a white pellet like stuff! I inverted the tubes a couple of times...

07 July 2016 4,730 6 View

Can I use a different unlabeled DNA probe as a specific competitor to the labeled DNA probe when performing EMSA?

I haven't got an unlabeled DNA probe of the same labelled (biotin) probe. I was wondering if it is mandatory to use the same unlabeled probe or can I use a different probe, which I am certain that...

06 June 2016 7,846 1 View