I have been growing SF9 cells on ex-cell media and have been trying to adapt them to grow on Grace's.
The media becomes extremely turbid after addition of 25% Grace's and 75% ex-cell.
How can I avoid this?
Forget Grace's use ES media :)
http://www.expressionsystems.com/
If you have formulation for ex-cell look for incompatible reagents (e.g. magnesium and carbonate) that will form precipitates. Some media types can be incompatible because of this...
In the past I've grown SF9s on Grace's medium without any issues.
We normally use Grace's medium or SF900 medium from Invitrogen for our cell culture without any issues.
Also are you growing attached or in suspension? Have you tried just spinning SF9s out of Ex-cell and resuspending in Grace's? Could be a bit harsh on cells - but you could check make up of media to see how similar they are?
I am growing attached, We have same Grace's from Invitrogen and we have plenty! and not as much Ex-cell so I have to figure out a way to adapt them.
Thank you for your help.
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